Wang Li, Zou Rong, Cai Jinghang, Liu Guihua, Jiang Ya, Chai Guanqun, Qin Song, Fan Chengwu
Institute of Soil and Fertilizer, Guizhou Academy of Agricultural Sciences, Guiyang 550006, China.
College of Forestry,Guizhou University, Guiyang 550025,China.
Heliyon. 2023 May 30;9(6):e16270. doi: 10.1016/j.heliyon.2023.e16270. eCollection 2023 Jun.
In order to evaluate the effect of intercropping of the hyperaccumulator, L. and wheat on the absorption and accumulation of cadmium (Cd) in wheat. The experiment was conducted with three replicates, which conducted on four Cd concentrations (0, 20, 40, 60 μmol L) in the Hoagland solution and using two planting patterns [monoculture wheat (MW), intercropping wheat and L. (IW，IS)]. The results showed that the addition of Cd in the solutions reduced the total root length by 19.08-55.98%, total root area by 12.35-44.48%, and total root volume by 16.01-46.00% of wheat plants. Intercropping with L. significantly reduced Cd contents by 28.3-47.2% and Cd accumulations by 10.08-32.43% in the roots of wheat. Transmission electron microscope (TEM) revealed that the root-tip cells of the monoculture wheat treated with Cd exhibited swollen spheres of intracellular mitochondria, disorderly arranged inner ridges of mitochondria, some damaged mitochondrial membranes, and deformed nuclear membranes. Many dense electron particles in the form of Cd were deposited in the cell gap, and the cell nucleus became smaller or even disappeared. Under the same Cd concentrations, root-tip cells of intercropped wheat showed much less density of electron particles, starch granules, and the damage to the nucleus and nuclear membrane by Cd.These results indicated that intercropping with L. reduced the Cd toxicity to wheat roots and decreased Cd uptake and accumulation in both the shoots and roots of the wheat.
为了评估超富集植物龙葵与小麦间作对小麦中镉（Cd）吸收和积累的影响。该实验设置了三个重复，在含有四种镉浓度（0、20、40、60 μmol/L）的霍格兰溶液中进行，并采用两种种植模式[单作小麦（MW）、小麦与龙葵间作（IW，IS）]。结果表明，溶液中添加镉使小麦植株的总根长减少了19.08 - 55.98%，总根面积减少了12.35 - 44.48%，总根体积减少了16.01 - 46.00%。与龙葵间作显著降低了小麦根中镉含量28.3 - 47.2%，镉积累量降低了10.08 - 32.43%。透射电子显微镜（TEM）显示，镉处理的单作小麦根尖细胞内线粒体呈肿胀球状，线粒体内嵴排列紊乱，部分线粒体膜受损，核膜变形。细胞间隙中有许多以镉形式存在的致密电子颗粒沉积，细胞核变小甚至消失。在相同镉浓度下，间作小麦根尖细胞中电子颗粒密度、淀粉粒以及镉对细胞核和核膜的损伤要少得多。这些结果表明，与龙葵间作降低了镉对小麦根的毒性，并减少了小麦地上部和根部对镉的吸收和积累。
