Ramshaw I A, Badenoch-Jones P, Grant A, Maxted M, Claudianos C
Invasion Metastasis. 1986;6(3):133-44.
Highly metastatic cell lines have been isolated from lung foci formed by the intravenous injection of large numbers (5 X 10(6)) of cells of a poorly metastatic rat mammary adenocarcinoma (DMBA-8). The metastatic variant lines were phenotypically different and, unlike the parent line, produced high levels of plasminogen activator (PA) separable into three major bands (MW 30,000, 48,000 and 83,000) on SDS-PAGE. It is suggested that PA may play a role in the enhanced metastatic ability of these cells. Using parallel DMBA-8 clones the rate of generation of the metastatic variant cells was estimated, by fluctuation analysis, to be approximately 1.85 X 10(-6) per cell generation.
通过静脉注射大量（5×10⁶）低转移性大鼠乳腺腺癌（DMBA - 8）细胞形成肺转移灶后，已从中分离出高转移性细胞系。这些转移变异细胞系在表型上有所不同，与亲代细胞系不同的是，它们产生高水平的纤溶酶原激活物（PA），在SDS - PAGE上可分离成三条主要条带（分子量分别为30,000、48,000和83,000）。提示PA可能在这些细胞增强的转移能力中起作用。利用平行的DMBA - 8克隆，通过波动分析估计转移变异细胞的产生率约为每细胞代1.85×10⁻⁶。
