Badenoch-Jones P, Claudianos C, Ramshaw I A
Department of Experimental Pathology, John Curtin School of Medical Research, Australian National University, Canberra City.
Invasion Metastasis. 1987;7(5):284-96.
Lectin-binding characteristics of a previously described highly metastatic variant (clone 4), derived in vivo from a poorly metastatic rat mammary adenocarcinoma (DMBA-8), have been investigated. of the lectins studied clone 4 cells, unlike the parent cells, bound Ulex europaeus agglutinin (UEA-1; specificity alpha-L-fucose) and peanut agglutinin (PNA; specificity D-galactose). These differences may be related to the greatly enhanced ability of clone 4 cells to form lung foci after intravenous injection. After neuraminidase treatment the differential binding of PNA, as shown by flow cytofluorography, was abrogated whereas that of UEA was unchanged. After separation by SDS-PAGE, four proteins in total cell extracts of clone 4 cells bound 125I-UEA applied to the gels. These had subunit molecular weights greater than 100,000 daltons and were also found in cellular extracts of another highly metastatic rat mammary adenocarcinoma (MAT 13762-B), but were missing from DMBA-8 cell extracts. In clone 4 and MAT 13762-B cells exogenous 3H-fucose was mainly incorporated into four fucoproteins of similar molecular weights to those which bound 125I-UEA. DMBA-8 cells, which incorporated slightly less exogenous fucose, showed a different pattern of fucoprotein labelling, which would seem to explain why DMBA-8 cells failed to bind UEA. Differences in cell surface protein iodination patterns were also noted between DMBA-8 and clone 4 cells.
对先前描述的一种高转移性变体（克隆4）的凝集素结合特性进行了研究，该变体是从低转移性大鼠乳腺腺癌（DMBA - 8）体内衍生而来的。在所研究的凝集素中，克隆4细胞与亲代细胞不同，它能结合欧洲荆豆凝集素（UEA - 1；特异性为α - L - 岩藻糖）和花生凝集素（PNA；特异性为D - 半乳糖）。这些差异可能与克隆4细胞静脉注射后形成肺转移灶的能力大大增强有关。经神经氨酸酶处理后，流式细胞荧光术显示的PNA差异结合被消除，而UEA的差异结合未改变。经SDS - PAGE分离后，克隆4细胞总细胞提取物中的四种蛋白质与凝胶上的125I - UEA结合。这些蛋白质的亚基分子量大于100,000道尔顿，在另一种高转移性大鼠乳腺腺癌（MAT 13762 - B）的细胞提取物中也有发现，但在DMBA - 8细胞提取物中缺失。在克隆4和MAT 13762 - B细胞中，外源性3H - 岩藻糖主要掺入四种分子量与结合125I - UEA的蛋白质相似的岩藻糖蛋白中。掺入外源性岩藻糖略少的DMBA - 8细胞显示出不同的岩藻糖蛋白标记模式，这似乎可以解释为什么DMBA - 8细胞不能结合UEA。在DMBA - 8和克隆4细胞之间还注意到细胞表面蛋白质碘化模式的差异。
