Nurhapsari Arlina, Cilmiaty Risya, Prayitno Adi, Purwanto Bambang, Soetrisno Soetrisno
Doctoral Degree of Medical Science, Faculty of Medicine, Sebelas Maret University, Surakarta, Central Java, Indonesia.
Department of Conservative Dentistry, Faculty of Dentistry, Islam Sultan Agung University, Semarang, Central Java, Indonesia.
Clin Cosmet Investig Dent. 2023 Jun 13;15:109-119. doi: 10.2147/CCIDE.S408158. eCollection 2023.
Acute dental pulp inflammation necessitates early treatment to alleviate inflammation and pain. In the inflammatory phase, a substance is required to lower the inflammatory mediators and reactive oxygen species that play a crucial role in that phase. Asiatic acid is a natural triterpene obtained from the plant with a high antioxidant value. This study examined the effect of Asiatic acid's antioxidant, anti-inflammatory, and antinociceptive properties on dental pulp inflammation.
The research is an experimental laboratory, with a post-test only with a control group design. The study utilised 40 male Wistar rats weighing 200-250 grams and aged 8-10 weeks. Rats were divided into five groups (control, eugenol, Asiatic Acid 0.5%; 1%; 2% group). Dental pulp inflammation was created in the maxillary incisor after six hours of administration of lipopolysaccharides (LPS). The dental pulp treatment then continued with the administration of eugenol and three different Asiatic acid concentrations (0.5%, 1% and 2%). In the next 72 hours, the teeth were biopsied, and the dental pulp was analysed using the enzyme-linked immunosorbent assay (ELISA) to measure the level of MDA, SOD, TNF-α, beta-endorphins and CGRP. Histopathological examination and the Rat Grimace Scale were utilised to determine the level of inflammation and pain, respectively.
The effect of Asiatic Acid on MDA, TNF-α, and CGRP levels decreased significantly compared to the control group (p=<0.001). On the SOD and beta-endorphin levels, Asiatic acid treatment resulted in a considerable rise (p =<0.001).
Due to its antioxidant, anti-inflammatory, and antinociceptive characteristics, Asiatic acid can reduce inflammation and pain in acute pulp inflammation due to its ability to decrease MDA, TNFα, and CGRP levels while raising SOD and beta-endorphin levels.
急性牙髓炎需要早期治疗以减轻炎症和疼痛。在炎症阶段，需要一种物质来降低在该阶段起关键作用的炎症介质和活性氧。积雪草苷是一种从植物中提取的具有高抗氧化价值的天然三萜。本研究考察了积雪草苷的抗氧化、抗炎和镇痛特性对牙髓炎的影响。
本研究为实验性实验室研究，采用仅设对照组的后测设计。研究使用了40只体重200 - 250克、年龄8 - 10周的雄性Wistar大鼠。大鼠被分为五组（对照组、丁香酚组、0.5%积雪草苷组、1%积雪草苷组、2%积雪草苷组）。在给予脂多糖（LPS）6小时后，在上颌切牙制造牙髓炎。然后继续用丁香酚和三种不同浓度的积雪草苷（0.5%、1%和2%）进行牙髓治疗。在接下来的72小时内，对牙齿进行活检，并使用酶联免疫吸附测定（ELISA）分析牙髓，以测量丙二醛（MDA）、超氧化物歧化酶（SOD）、肿瘤坏死因子-α（TNF-α）、β-内啡肽和降钙素基因相关肽（CGRP）的水平。分别利用组织病理学检查和大鼠面部表情量表来确定炎症和疼痛程度。
与对照组相比，积雪草苷对MDA、TNF-α和CGRP水平的影响显著降低（p =<0.001）。在SOD和β-内啡肽水平上，积雪草苷治疗导致显著升高（p =<0.001）。
由于其抗氧化、抗炎和镇痛特性，积雪草苷能够降低MDA、TNFα和CGRP水平，同时提高SOD和β-内啡肽水平，从而减轻急性牙髓炎的炎症和疼痛。
