College of Medicine and Pharmacy, Duy Tan University, Danang, Vietnam.
Department of Neurosurgery, Hue University Hospital, Hue University of Medicine and Pharmacy, Hue University, Hue, Vietnam.
J Biomol Struct Dyn. 2024 Jul;42(10):5114-5127. doi: 10.1080/07391102.2023.2225109. Epub 2023 Jun 19.
Coronary heart disease (CHD) is a prevalent global cause of death. Research suggests that circular RNAs (circRNAs) play a role in the development of CHD. In this study, we investigated the expression of hsa_circRNA_0000284 in peripheral blood leukocytes (PBLs) obtained from a cohort of 94 CHD patients aged over 50 years, as well as 126 age-matched healthy controls (HC). An inflammatory and oxidative injury cell model that simulates CHD was used to evaluate changes in hsa_ circRNA 0000284 under stress. CRISPR/Cas9 technology was used to evaluate changes in hsa_circRNA_0000284 expression. An hsa circRNA_0000284 overexpression and silencing cell model was used to analyze the biological functions of hsa_circRNA_0000284. Bioinformatics, qRT-PCR, viral transfection technology, and luciferase assays were used to evaluate the potential hsa_circRNA_0000284/miRNA-338-3p/ axis. Western blotting analysis was performed to detect protein expression. Herein, PBLs from CHD patients exhibited downregulation of hsa_circRNA_0000284 expression. Exposure to oxidative stress and inflammation can induce damage to human umbilical endothelial cells, resulting in the downregulation of hsa_circRNA_0000284 expression. The expression of hsa_circRNA_0000284 in EA-hy926 cells was significantly reduced after the element of hsa_circRNA_0000284 had been knocked out. The expression of hsa_circRNA_0000284 affected proliferation, cycle distribution, aging, and apoptosis in EA-hy926 cells. Consistent with the results of cell transfection experiments and luciferase assays, Western blotting showed that hsa_circRNA_0000284 plays a role in the regulation of hsa-miRNA-338-3p expression. Subsequently, hsa-miRNA-338-3p was found to be involved in the regulation of ETS1 expression.Communicated by Ramaswamy H. Sarma.
冠心病（CHD）是一种普遍存在的全球致死原因。研究表明，环状 RNA（circRNA）在 CHD 的发展中发挥作用。在这项研究中，我们调查了来自 94 名年龄超过 50 岁的 CHD 患者和 126 名年龄匹配的健康对照者（HC）的外周血白细胞（PBL）中 hsa_circRNA_0000284 的表达。使用模拟 CHD 的炎症和氧化损伤细胞模型来评估应激下 hsa_ circRNA 0000284 的变化。CRISPR/Cas9 技术用于评估 hsa_circRNA_0000284 表达的变化。使用 hsa circRNA_0000284 过表达和沉默细胞模型来分析 hsa_circRNA_0000284 的生物学功能。生物信息学、qRT-PCR、病毒转染技术和荧光素酶测定用于评估潜在的 hsa_circRNA_0000284/miRNA-338-3p/轴。Western 印迹分析用于检测蛋白表达。在此，CHD 患者的 PBL 表现出 hsa_circRNA_0000284 表达下调。氧化应激和炎症的暴露会导致人脐静脉内皮细胞损伤，从而导致 hsa_circRNA_0000284 表达下调。在敲除 hsa_circRNA_0000284 元件后，EA-hy926 细胞中 hsa_circRNA_0000284 的表达显著降低。hsa_circRNA_0000284 的表达影响 EA-hy926 细胞的增殖、周期分布、衰老和凋亡。与细胞转染实验和荧光素酶测定的结果一致，Western 印迹显示 hsa_circRNA_0000284 在调节 hsa-miRNA-338-3p 表达中起作用。随后发现 hsa-miRNA-338-3p 参与调节 ETS1 表达。由 Ramaswamy H. Sarma 交流。
