Department of Toxicology and Nutrition, School of Public Health, Cheeloo College of Medicine, Shandong University, Jinan, China.
Department of Nutrition, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, China.
Nutr Cancer. 2023;75(8):1673-1686. doi: 10.1080/01635581.2023.2223789. Epub 2023 Jun 19.
The previous documentation has shown the role of resistant starch in promoting intestinal health, while the effect of starch-lipid complex (RS5) on colitis remains unclear. This study aimed to investigate the effect and potential mechanism of RS5 in colitis. We prepared RS5 complexes by combining pea starch with lauric acid. Mice with dextran sulfate sodium-induced colitis were treated with either RS5 (3.25 g/kg) or normal saline (10 mL/kg) for seven days, and the effects of pea starch-lauric acid complex on mice were observed. The RS5 treatment significantly attenuated weight loss, splenomegaly, colon shortening, and pathological damage in mice with colitis. Compare with the DSS group, cytokines levels, such as tumor necrosis factor-α and interleukin-6 in both serum and colon tissue was significantly decreased in RS5 treatment group, while the gene expression of and the expression of mucin 2, zonula occludens-1, Occludin, and claudin-1 in the colon was significantly upregulated in RS5 treatment group. In addition, RS5 treatment altered the gut microbiota structure of colitis mice by increasing the abundance of and decreasing , , , and . The dietary composition could be exploited to manage colitis by attenuating inflammation, restoring the intestinal barrier, and regulating gut microbiota.
先前的文献已经表明抗性淀粉在促进肠道健康方面的作用，而淀粉-脂质复合物（RS5）对结肠炎的影响尚不清楚。本研究旨在探讨 RS5 在结肠炎中的作用及潜在机制。我们通过将豌豆淀粉与月桂酸结合来制备 RS5 复合物。用葡聚糖硫酸钠诱导结肠炎的小鼠用 RS5（3.25 g/kg）或生理盐水（10 mL/kg）处理 7 天，观察豌豆淀粉-月桂酸复合物对小鼠的影响。RS5 治疗显著减轻了结肠炎小鼠的体重减轻、脾肿大、结肠缩短和病理损伤。与 DSS 组相比，RS5 治疗组血清和结肠组织中的肿瘤坏死因子-α和白细胞介素-6 等细胞因子水平显著降低，而结肠中 和粘蛋白 2、闭合蛋白-1、Occludin 和 Claudin-1 的基因表达显著上调。此外，RS5 治疗通过增加 的丰度和减少 、 、 、和 来改变结肠炎小鼠的肠道微生物群结构。可以通过改变饮食成分来管理结肠炎，减轻炎症、恢复肠道屏障和调节肠道微生物群。
