豌豆白蛋白通过调节 NF-κB 信号通路和肠道微生物群缓解葡聚糖硫酸钠诱导的结肠炎。
Pea Albumin Attenuates Dextran Sulfate Sodium-Induced Colitis by Regulating NF-κB Signaling and the Intestinal Microbiota in Mice.
机构信息
Key Laboratory of Precision Nutrition and Food Quality, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
Beijing Advanced Innovation Center for Food Nutrition and Human Health, Department of Nutrition and Health, China Agricultural University, Beijing 100193, China.
出版信息
Nutrients. 2022 Sep 1;14(17):3611. doi: 10.3390/nu14173611.
BACKGROUND
Inflammatory bowel disease remains a global burden with rapidly increasing incidence and prevalence in both industrialized countries and developing countries. In this study, we prepared pea albumin from pea seeds and determined its beneficial effects being anti-inflammatory and on gut microbiota modulation in dextran sulfate sodium (DSS)-challenged mice.
METHOD
Six-week-old C57BL/6N male mice received an equivalent volume (200 μL) of sterile phosphate balanced solution, 0.375, 0.75, or 1.50 g/kg body weight (BW) of pea albumin that was subjected to 2.0% DSS for 7 days to induce colitis. On day 17 of the experiment, all mice were sacrificed after blood sample collection, and colon tissue and colon contents were collected. BW change curve, colon length, myeloperoxidase (MPO) activity, mucus staining, immunofluorescence staining of T cells and macrophages, cytokines, pro-inflammatory genes expression, nuclear factor-κB (NF-κB) and signal transducer, and activator of transcription 3 (STAT3) signaling pathways as well as 16S DNA sequence were measured.
RESULTS
Our results show that pea albumin alleviates DSS-induced BW loss, colon length shortening, enhanced MPO activity, cytokines secretion, mucus deficiency, and inflammatory cell infiltration, as well as enhanced pro-inflammatory genes expression. In addition, the overactivation of NF-κB and STAT3 following DSS exposure is attenuated by pea albumin administration. Of particular interest, pea albumin oral administration restored gut microbiota dysbiosis as evidenced by enhanced α-diversity, restored β-diversity, and promoted relative abundance of and .
CONCLUSION
Taken together, the data provided herein demonstrated that pea albumin plays a protective role in DSS-induced colitis by reducing inflammatory cell infiltration, pro-inflammatory genes expression and pro-inflammatory cytokines release, inactivation of NF-κB signal, and gut microbiota modulation.
背景
炎症性肠病仍然是一个全球性的负担，在工业化国家和发展中国家的发病率和患病率都在迅速增加。在这项研究中，我们从豌豆种子中制备了豌豆白蛋白，并确定了其在葡聚糖硫酸钠（DSS）诱导的小鼠中的抗炎和调节肠道微生物群的有益作用。
方法
6 周龄 C57BL/6N 雄性小鼠接受等体积（200μL）无菌磷酸盐平衡溶液、0.375、0.75 或 1.50g/kg 体重（BW）的豌豆白蛋白，用 2.0%DSS 处理 7 天以诱导结肠炎。实验第 17 天，所有小鼠在采集血样后处死，收集结肠组织和结肠内容物。测量 BW 变化曲线、结肠长度、髓过氧化物酶（MPO）活性、粘液染色、T 细胞和巨噬细胞免疫荧光染色、细胞因子、促炎基因表达、核因子-κB（NF-κB）和信号转导和转录激活因子 3（STAT3）信号通路以及 16S DNA 序列。
结果
我们的结果表明，豌豆白蛋白减轻了 DSS 诱导的 BW 损失、结肠长度缩短、增强的 MPO 活性、细胞因子分泌、粘液缺乏和炎症细胞浸润，以及增强的促炎基因表达。此外，DSS 暴露后 NF-κB 和 STAT3 的过度激活被豌豆白蛋白的给药减弱。特别有趣的是，豌豆白蛋白口服给药恢复了肠道微生物群的失调，表现为α-多样性增强、β-多样性恢复，以及 和 的相对丰度增加。
结论
综上所述，本文提供的数据表明，豌豆白蛋白通过减少炎症细胞浸润、促炎基因表达和促炎细胞因子释放、NF-κB 信号失活以及调节肠道微生物群，在 DSS 诱导的结肠炎中发挥保护作用。
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