KingMed Center for Clinical Laboratory Co., Ltd, Hangzhou, Zhejiang Province, China.
Taizhou Traditional Chinese Medicine Hospital, Taizhou, Jiangsu Province, China.
Medicine (Baltimore). 2023 Jun 9;102(23):e34019. doi: 10.1097/MD.0000000000034019.
Lung cancer in never smokers (LCNS) has been considered as a separate disease and the 7th cause of cancer-related death worldwide. However, limited research has focused on "female" cohorts, which have presented a higher incidence rate. In this study, the microarray data of lung cancer tissues derived from 54 female lung cancer patients, consisting of 43 nonsmokers and 11 smokers, were selected from GSE2109 dataset. A total of 249 differentially expressed genes (DEGs) including 102 up- and 147 down-regulated genes were identified and further analyzed for gene ontology (GO) terms and Kyoto encyclopedia of genes and genomes (KEGG) pathway enrichment. By constructing protein-protein interaction (PPI) network and calculating key modules, 10 hub genes were screened out. The module analysis of the PPI network presented that the progression of female LCNS was significantly associated with immune response as chemokine activity and lipopolysaccharide response, and these biological processes (BP) might be mediated by chemokine signaling pathway and cytokine-cytokine receptor interaction. Then, survival analysis by Kaplan-Meier (K-M) Plotter online platform presented down-regulated gene colony stimulating factor 2 receptor beta common subunit (CSF2RB) of female LCNS might be involved in poor clinical outcome. Female LCNS with high expression of CSF2RB might be relevant with relative risk reduction of mortality, longer median survival time and higher 5-year survival rate, while female LCNS with low expression of CSF2RB might be implicated in a poor clinical outcome. In short, our results support CSF2RB to be a candidate survival predictor for female LCNS.
不吸烟人群肺癌（LCNS）已被视为一种独立的疾病，也是全球第七大癌症相关死亡原因。然而，针对“女性”队列的研究有限，这些队列的发病率更高。在这项研究中，从 GSE2109 数据集选择了 54 名女性肺癌患者的肺癌组织的微阵列数据，包括 43 名不吸烟者和 11 名吸烟者。共鉴定出 249 个差异表达基因（DEGs），包括 102 个上调基因和 147 个下调基因，并进一步分析其基因本体（GO）术语和京都基因与基因组百科全书（KEGG）通路富集。通过构建蛋白质-蛋白质相互作用（PPI）网络并计算关键模块，筛选出 10 个枢纽基因。PPI 网络的模块分析表明，女性 LCNS 的进展与免疫反应密切相关，如趋化因子活性和脂多糖反应，这些生物学过程（BP）可能由趋化因子信号通路和细胞因子-细胞因子受体相互作用介导。然后，通过 Kaplan-Meier（K-M）Plotter 在线平台进行生存分析表明，女性 LCNS 下调基因集落刺激因子 2 受体β共同亚基（CSF2RB）可能与不良临床结局有关。CSF2RB 高表达的女性 LCNS 可能与死亡率相对风险降低、中位生存时间延长和 5 年生存率提高相关，而 CSF2RB 低表达的女性 LCNS 可能与不良临床结局有关。总之，我们的研究结果支持 CSF2RB 作为女性 LCNS 的候选生存预测因子。
