Department of Obstetrics & Gynecology, Tongji Hospital, Tongji Medical College, Huazhong University of Science & Technology, Hubei Province, China.
Medicine (Baltimore). 2023 Jun 9;102(23):e33911. doi: 10.1097/MD.0000000000033911.
Similarities between luteinized thecoma associated with sclerosing peritonitis (LTSP) and thecoma, cause difficulty in clinical differential diagnoses. To improve the situation, we selected 10 specified molecular pathological markers that are frequently used in clinical pathology of ovarian sex cord-stromal tumors to determine whether they exert a discriminatory effect.
Applying immunohistochemistry, we analyzed the expression of alpha-1,6-mannosylglycoprotein 6-beta-n-acetylglucosaminyltransferase B (MGAT5B), nuclear receptor coactivator 3 (NCOA3), proliferation marker protein Ki-67 (MKI67), estrogen receptor, progesterone receptor, Vimentin, receptor tyrosine-protein kinase erbB-2, Catenin beta-1 (β-Catenin), CD99 antigen (CD99) and Wilms tumor protein (WT1) in 102 cases of diseases containing 11 LTSP and 91 thecoma. Whole-exome sequencing and fluorescence in situ hybridization were used to examine the MGAT5B-NCOA3 fusion gene in LTSP. Statistical analysis was performed using t test, one-way analysis of variance test, and post hoc test.
Six significant markers were verified for the discrimination between LTSP and thecoma, containing 4 upregulating indicators MGAT5B, NCOA3, MKI67, β-Catenin, and 2 downregulating markers CD99 and WT1 in luteinized cells. In addition, the MGAT5B-NCOA3 fusion gene was identified in LTSP for the first time with significantly rich expression compared to thecoma.
We verified 6 significant molecular pathological markers containing MGAT5B, NCOA3, MKI67, β-Catenin, CD99, and WT1 and identified MGAT5B-NCOA3 fusion gene in LTSP; this work will help clinicians to discriminate between medical conditions and treat patients accurately.
黄体化间质细胞瘤伴硬化性腹膜炎（LTSP）与间质细胞瘤之间存在相似性，这给临床鉴别诊断带来了困难。为改善这种情况，我们选择了 10 种常用于卵巢性索-间质肿瘤临床病理的特定分子病理标志物，以确定它们是否具有鉴别作用。
应用免疫组织化学方法，我们分析了 11 例 LTSP 和 91 例间质细胞瘤中 102 例疾病中α-1,6-甘露糖基转移酶 6-β-N-乙酰氨基葡萄糖基转移酶 B（MGAT5B）、核受体共激活因子 3（NCOA3）、增殖标志物蛋白 Ki-67（MKI67）、雌激素受体、孕激素受体、波形蛋白、受体酪氨酸蛋白激酶 erbB-2、连接蛋白β-1（β-Catenin）、CD99 抗原（CD99）和 Wilms 肿瘤蛋白（WT1）的表达。应用全外显子测序和荧光原位杂交技术检测 LTSP 中的 MGAT5B-NCOA3 融合基因。采用 t 检验、单因素方差分析及事后检验进行统计学分析。
验证了 6 个用于区分 LTSP 和间质细胞瘤的显著标志物，包括黄体化细胞中 4 个上调标志物 MGAT5B、NCOA3、MKI67、β-Catenin 和 2 个下调标志物 CD99 和 WT1。此外，首次在 LTSP 中发现 MGAT5B-NCOA3 融合基因，其表达明显比间质细胞瘤丰富。
我们验证了 6 个具有显著意义的分子病理标志物，包括 MGAT5B、NCOA3、MKI67、β-Catenin、CD99 和 WT1，并在 LTSP 中发现了 MGAT5B-NCOA3 融合基因；这项工作将有助于临床医生进行鉴别诊断，准确治疗患者。
