Fan Wen-Jie, Zhou Meng-Xi, Wang Di-Di, Jiang Xin-Xin, Ding Hao
The First Affiliated Hospital of Anhui Medical University, Department of Radiology, Hefei, Anhui Province, China.
The First Affiliated Hospital of Anhui Medical University, Department of Gastroenterology, Hefei, Anhui Province, China.
Genet Mol Biol. 2023 Jun 16;46(2):e20220323. doi: 10.1590/1678-4685-GMB-2022-0323. eCollection 2023.
Hepatocellular carcinoma (HCC) is the most common type of liver malignancy with high incidence and poor prognosis. Transmembrane protein 147 (TMEM147) has been implicated in the development of colon cancer. However, the role of TMEM147 in HCC remains unclear. In this study, data of 371 HCC tissues, 50 adjacent nontumor tissues, and 110 normal liver tissues were retrieved from the TCGA and GTEx databases. TMEM147 expression was found to be increased in HCC tissues. High expression of TMEM147 was related to poor prognosis, and TMEM147 was confirmed to be an independent prognostic factor for HCC patients. A receiver operating characteristics (ROC) analysis was performed and showed that the diagnostic efficacy of TMEM147 was significantly higher than that of AFP (0.908 versus 0.746, p < 0.001). Furthermore, TMEM147 promoted tumor immune infiltration, and macrophages were the immune cells that predominantly expressed TMEM147 in HCC. Further analysis revealed that TMEM147 mainly impacted the ribosome pathway, and CTCF, MLLT1, TGIF2, ZNF146, and ZNF580 were predicted to be the upstream transcription factors for TMEM147 in HCC. These results suggest that TMEM147 serves as a promising biomarker for diagnosis and prognosis and may potentially become a therapeutic target for HCC.
肝细胞癌（HCC）是最常见的肝脏恶性肿瘤类型，发病率高且预后较差。跨膜蛋白147（TMEM147）与结肠癌的发生有关。然而，TMEM147在HCC中的作用仍不清楚。在本研究中，从TCGA和GTEx数据库中检索了371例HCC组织、50例癌旁非肿瘤组织和110例正常肝组织的数据。发现HCC组织中TMEM147表达增加。TMEM147高表达与预后不良相关，并且TMEM147被证实是HCC患者的独立预后因素。进行了受试者工作特征（ROC）分析，结果显示TMEM147的诊断效能显著高于甲胎蛋白（AFP）（0.908对0.746，p<0.001）。此外，TMEM147促进肿瘤免疫浸润，巨噬细胞是HCC中主要表达TMEM147的免疫细胞。进一步分析显示TMEM147主要影响核糖体途径，并且CTCF、MLLT1、TGIF2、ZNF146和ZNF580被预测为HCC中TMEM147的上游转录因子。这些结果表明，TMEM147作为一种有前景的诊断和预后生物标志物，可能成为HCC的治疗靶点。
