Medical Center of Hematology, The Second Affiliated Hospital, Army Medical University, Chongqing, 40037, China; State Key Laboratory of Trauma, Burn and Combined Injury, Army Medical University, Chongqing, 40037, China.
Medical Center of Hematology, The Second Affiliated Hospital, Army Medical University, Chongqing, 40037, China; State Key Laboratory of Trauma, Burn and Combined Injury, Army Medical University, Chongqing, 40037, China; Jinfeng Laboratory, Chongqing, 401329, China.
Cancer Lett. 2023 Jul 28;567:216281. doi: 10.1016/j.canlet.2023.216281. Epub 2023 Jun 17.
Clonal evolution has gained immense attention in explaining cancer cell status, history, and fate during cancer progression. Current single-cell or spatial transcriptome technologies have broadened our understanding of various mechanisms underlying cancer initiation, relapse, and drug resistance. However, technical challenges still hinder a better understanding of the dynamics of distinctive phenotypic states and abnormal trajectories from normal physiological transition to malignant stages. Cellular barcoding enabled lineage tracing on parallelly massive cells at single-cell resolution through different mechanisms lately, enabling new insights into exploring developmental trajectories, cancer progression, and targeted therapies. This review summarizes the latest noteworthy and robust strategies for different types of cellular barcodes. To introduce the major characteristics, advantages and limitations of these different strategies, this review will further guide in choosing or improving cellular barcoding technologies and their applications in cancer research.
克隆进化在解释癌症进展过程中癌细胞的状态、历史和命运方面引起了极大的关注。目前的单细胞或空间转录组技术拓宽了我们对癌症发生、复发和耐药性背后各种机制的理解。然而，技术挑战仍然阻碍了我们更好地理解从正常生理转化到恶性阶段的独特表型状态和异常轨迹的动态。最近，细胞条形码通过不同的机制使并行大量细胞在单细胞分辨率上进行谱系追踪成为可能，为探索发育轨迹、癌症进展和靶向治疗提供了新的见解。本综述总结了不同类型细胞条形码的最新显著和强大的策略。为了介绍这些不同策略的主要特点、优点和局限性，本综述将进一步指导选择或改进细胞条形码技术及其在癌症研究中的应用。
