Graduate Student (Master's), Biosciences and Pathophysiology Program, State University of Maringá, Maringá, Brazil.
Postgraduate student (PhD), Biosciences and Pathophysiology Program, State University of Maringá, Maringá, Brazil.
Photodiagnosis Photodyn Ther. 2023 Sep;43:103659. doi: 10.1016/j.pdpdt.2023.103659. Epub 2023 Jun 17.
Sporothrix brasiliensis is a pathogenic dimorphic fungus that affects humans and animals causing sporotrichosis. The treatment of this disease with conventional antifungals commonly results in therapeutic failures and resistance. Therefore, this study aimed to evaluate the in vitro effect of curcumin (CUR) mediated by photodynamic therapy (PDT) in its pure state and incorporated into pharmaceutical formulation in gel form, on the filamentous and yeast forms of S. brasiliensis.
Cells from both forms of the fungus were treated with pure curcumin (PDT-CUR). For this, CUR concentrations ranging from 0.09 to 50 μM were incubated for 15 min and then irradiated with blue LED at 15 J/cm². Similarly, it was performed with PDT-CUR-gel, at lower concentration with fungistatic action. After, a qualitative and quantitative (colony forming units (CFU)) analysis of the results was performed. Additionally, reactive oxygen species (ROS) were detected by flow cytometry. Results PDT with 0.78 μM of CUR caused a significant reduction (p < 0.05) in cells of the filamentous and yeast form, 1.38 log and 1.18 log, respectively, in comparison with the control. From the concentration of 1.56 μM of CUR, there was a total reduction in the number of CFU (≥ 3 log). The PDT-CUR-gel, in relation to its base without CUR, presented a significant reduction (p < 0.05) of 0.83 log for the filamentous form and for the yeast form, 0.72 log. ROS release was detected after the PDT-CUR assay, showing that this may be an important pathway of death caused by photoinactivation. Conclusion PDT-CUR has an important in vitro antifungal action against S. brasiliensis strains in both morphologies.
巴西利什曼原虫是一种致病性二态真菌，可感染人类和动物，导致孢子丝菌病。传统抗真菌药物治疗这种疾病通常会导致治疗失败和耐药性。因此，本研究旨在评估姜黄素（CUR）介导的光动力疗法（PDT）在纯态和凝胶态药物制剂中的体外抗巴西利什曼原虫丝状和酵母两种形态的效果。
用纯姜黄素（PDT-CUR）处理两种形态的真菌细胞。为此，将 CUR 浓度范围从 0.09 到 50 μM 孵育 15 分钟，然后用 15 J/cm²的蓝光 LED 照射。同样，在具有抑菌作用的较低浓度下，对 PDT-CUR-凝胶进行了同样的处理。然后，对结果进行定性和定量（菌落形成单位（CFU））分析。此外，通过流式细胞术检测活性氧（ROS）。结果：0.78 μM 的 CUR 的 PDT 导致丝状和酵母两种形态的细胞显著减少（p < 0.05），分别减少 1.38 对数和 1.18 对数，与对照组相比。从 1.56 μM 的 CUR 浓度开始，CFU 数量完全减少（≥ 3 对数）。与不含 CUR 的基础凝胶相比，PDT-CUR-凝胶使丝状形态减少了 0.83 对数，酵母形态减少了 0.72 对数，差异有统计学意义（p < 0.05）。在 PDT-CUR 试验后检测到 ROS 释放，表明这可能是光灭活引起死亡的一个重要途径。结论：PDT-CUR 对两种形态的巴西利什曼原虫菌株具有重要的体外抗真菌作用。
