Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Mansoura University, Mansoura, Egypt.
Luminescence. 2023 Sep;38(9):1572-1582. doi: 10.1002/bio.4539. Epub 2023 Jul 13.
Moxifloxacin and ofloxacin are two broad-spectrum quinolone antibiotics. They are among the most widely used antibiotics, at this time, applied to control the COVID-19 pandemic. Hydroxychloroquine is an FDA-approved drug for the treatment of COVID-19. This work describes a simple, green, selective, and sensitive spectrofluorimetric method for the assay of moxifloxacin and ofloxacin in the presence of hydroxychloroquine, two co-administered mixtures used in the treatment of hospital-acquired pneumonia in patients with COVID-19. Simultaneous assay of hydroxychloroquine and moxifloxacin was carried out in methanol using a direct spectrofluorimetric method (method I) at 375 and 550 nm, respectively, after excitation at 300 nm. The direct spectrofluorimetric assay was rectilinear over concentration ranges 50.0-400.0 and 300.0-2500.0 ng/ml for hydroxychloroquine and moxifloxacin, respectively, with limits of detection (LOD) of 6.4 and 33.64 ng/ml and limits of quantitation (LOQ) of 19.4 and 102.6 ng/ml, respectively, for the two drugs. The assay for hydroxychloroquine and ofloxacin was carried out by measuring the first derivative synchronous amplitude for hydroxychloroquine at the zero crossing point of ofloxacin and vice versa at Δλ = 140 nm (method II). Hydroxychloroquine was measured at 266 nm, while ofloxacin was measured at 340 nm over the concentration range 4-40 ng/ml for hydroxychloroquine and 200-2000 ng/ml for ofloxacin with LOD of 0.467 and 25.3 ng/ml and LOQ of 1.42 and 76.6 ng/ml, respectively, for the two drugs. The two methods were validated following International Conference on Harmonization guidelines and were applied to the analysis of the two drugs in plasma with good percentage recoveries (109.73-93.17%).
莫西沙星和氧氟沙星是两种广谱喹诺酮类抗生素。它们是目前应用最广泛的抗生素之一，用于控制 COVID-19 大流行。羟氯喹是一种获得 FDA 批准的用于治疗 COVID-19 的药物。本工作描述了一种简单、绿色、选择性和灵敏的分光荧光法，用于测定莫西沙星和氧氟沙星在羟氯喹存在下的含量，这两种药物联合用于治疗 COVID-19 患者医院获得性肺炎。在甲醇中，使用直接分光荧光法（方法 I），分别在 300nm 激发下于 375nm 和 550nm 处测定羟氯喹和莫西沙星的同时含量。直接分光荧光法在羟氯喹和莫西沙星的浓度范围内分别为 50.0-400.0 和 300.0-2500.0ng/ml 时呈线性，羟氯喹和莫西沙星的检测限（LOD）分别为 6.4 和 33.64ng/ml，定量限（LOQ）分别为 19.4 和 102.6ng/ml。测定羟氯喹和氧氟沙星的方法是通过测量羟氯喹在氧氟沙星零交点处的一阶导数同步幅度，并反之测量氧氟沙星在羟氯喹处的一阶导数同步幅度，在 Δλ=140nm 处（方法 II）。羟氯喹在 266nm 处测定，氧氟沙星在 340nm 处测定，羟氯喹的浓度范围为 4-40ng/ml，氧氟沙星的浓度范围为 200-2000ng/ml，羟氯喹和氧氟沙星的检测限分别为 0.467 和 25.3ng/ml，定量限分别为 1.42 和 76.6ng/ml。两种方法均按照国际协调会议指南进行了验证，并应用于血浆中两种药物的分析，回收率良好（109.73-93.17%）。
