不产生四环素霉素C的淡青链霉菌突变体的分离
Isolation of tetracenomycin C-nonproducing Streptomyces glaucescens mutants.
作者信息
Motamedi H, Wendt-Pienkowski E, Hutchinson C R
出版信息
J Bacteriol. 1986 Aug;167(2):575-80. doi: 10.1128/jb.167.2.575-580.1986.
Abstract
We analyzed the properties of tetracenomycin C (TcmC)-nonproducing mutants of Streptomyces glaucescens to establish the nature of pathway intermediates and to provide some information about the genetics of antitumor anthracycline antibiotic production. Using cosynthesis properties and metabolite accumulation data, we classified a collection of 34 TcmC-nonproducing strains into seven different groups. From this information, we deduced the positions of the tcm mutations in relation to a hypothetical TcmC biosynthetic pathway and suggest which pathway enzymes are affected by the different mutations.
摘要
我们分析了青灰链霉菌四环霉素C（TcmC）非产生突变体的特性，以确定途径中间体的性质，并提供一些关于抗肿瘤蒽环类抗生素生产遗传学的信息。利用共合成特性和代谢物积累数据，我们将34株TcmC非产生菌株分为七个不同的组。根据这些信息，我们推断了tcm突变相对于假设的TcmC生物合成途径的位置，并指出了哪些途径酶受不同突变的影响。
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