Shen B, Nakayama H, Hutchinson C R
School of Pharmacy, University of Wisconsin, Madison 53706.
J Nat Prod. 1993 Aug;56(8):1288-93. doi: 10.1021/np50098a013.
This report describes the fermentation, isolation, and structural elucidation of tetracenomycin (Tcm) F2 [2], a metabolite produced by a blocked mutant strain WMH1092 of the Tcm C [1] producer Streptomyces glaucescens and by the recombinant strain S. glaucescens WMH1077 (pWHM722). Elucidation of the Tcm F2 structure shows that 2 is the earliest intermediate identified to date in the biosynthesis of 1. This is supported by the fact that 2 is very efficiently biotransformed to 1 by the S. glaucescens WMH1068 strain and is enzymatically converted to Tcm F1 [3] and to Tcm D3 [4], a known intermediate of Tcm C biosynthesis.
本报告描述了四环素霉素（Tcm）F2 [2] 的发酵、分离及结构解析，Tcm F2是由Tcm C [1] 产生菌蓝灰色链霉菌的阻断突变株WMH1092以及重组菌株蓝灰色链霉菌WMH1077（pWHM722）产生的一种代谢产物。Tcm F2结构的解析表明，化合物2是迄今为止在1的生物合成中鉴定出的最早中间体。这一点得到了以下事实的支持：化合物2能被蓝灰色链霉菌WMH1068菌株高效地生物转化为1，并能被酶促转化为Tcm F1 [3] 和Tcm D3 [4]，Tcm D3是Tcm C生物合成的一个已知中间体。
