Hintermann G, Crameri R, Vögtli M, Hütter R
Mol Gen Genet. 1984;196(3):513-20. doi: 10.1007/BF00436201.
The wild type strain of Streptomyces glaucescens produces hydroxystreptomycin and has a natural resistance towards the streptomycin group aminoglycoside antibiotics. The inherent resistance is a genetically unstable character and mutant strains sensitive to streptomycins arise spontaneously at unusually high frequencies. The gene conferring streptomycin resistance was cloned and characterised as a streptomycin specific phosphotransferase. Hybridisation experiments show that the mutational event leading to sensitivity is due to large deletions, most likely on the chromosome, comprehending the structural gene coding for a streptomycin phosphotransferase and its flanking regions. Interspecific expression of the S. glaucescens phosphotransferase was found in Streptomyces lividans as well as in Escherichia coli.
青紫链霉菌的野生型菌株产生羟基链霉素，并对链霉素类氨基糖苷抗生素具有天然抗性。这种固有抗性是一种遗传不稳定的特性，对链霉素敏感的突变菌株会以异常高的频率自发产生。赋予链霉素抗性的基因被克隆并鉴定为链霉素特异性磷酸转移酶。杂交实验表明，导致敏感性的突变事件是由于大片段缺失，最有可能发生在染色体上，包括编码链霉素磷酸转移酶的结构基因及其侧翼区域。在天蓝色链霉菌以及大肠杆菌中发现了青紫链霉菌磷酸转移酶的种间表达。
