Departamento de Microbiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, C.P. 62210, Cuernavaca, Morelos, México.
Instituto de Ciencias Físicas, Universidad Nacional Autónoma de México, C.P. 62210, Cuernavaca, Morelos, México.
J Antibiot (Tokyo). 2023 Oct;76(10):603-612. doi: 10.1038/s41429-023-00637-8. Epub 2023 Jun 19.
Currently, antibiotic-resistant bacteria represent a serious threat to public health worldwide. Biofilm formation potentiates both virulence and antibiotic resistance of bacteria. Therefore, the discovery of new antibacterial and antibiofilm compounds is an issue of paramount importance to combat and prevent hard-to-treat bacterial infections. Zeolitic-imidazolate-frameworks (ZIFs) are metallo-organic compounds known to have various interesting chemical and biological applications, including antibacterial properties. In this study, we synthesized ZIF-67 nanoparticles, formed by imidazolate anions and cobalt cations, and found that they inhibit the growth of Acinetobacter baumannii, Pseudomonas aeruginosa, and Staphylococcus aureus. Sub-inhibitory concentrations of ZIF-67 were also able to significantly reduce the biomass of pre-established biofilms of these pathogenic bacteria. On the other hand, the ZIF-67 nanoparticles had null or low cytotoxicity in mammalian cells at those concentrations showing antibacterial or antibiofilm activities. Thus, our results reveal the potential of ZIF-67 nanoparticles to be used against pathogenic bacteria.
目前，抗生素耐药菌对全球公共卫生构成严重威胁。生物膜的形成增强了细菌的毒力和抗生素耐药性。因此，发现新的抗菌和抗生物膜化合物是对抗和预防难以治疗的细菌感染的头等大事。沸石咪唑酯骨架（ZIFs）是一种金属有机化合物，已知具有多种有趣的化学和生物学应用，包括抗菌性能。在这项研究中，我们合成了由咪唑阴离子和钴阳离子组成的 ZIF-67 纳米颗粒，发现它们可以抑制鲍曼不动杆菌、铜绿假单胞菌和金黄色葡萄球菌的生长。亚抑制浓度的 ZIF-67 也能显著减少这些病原菌已建立的生物膜的生物量。另一方面，在显示出抗菌或抗生物膜活性的浓度下，ZIF-67 纳米颗粒对哺乳动物细胞的细胞毒性为零或低。因此，我们的结果表明 ZIF-67 纳米颗粒具有对抗病原菌的潜力。
