Antwi-Baah Ruth, Acquah Mirabel Ewura Esi, Dapaah Malcom Frimpong, Chen Xiaoqin, Walker Joojo, Liu Heyang
School of Physical Science and Technology, Sichuan University, Chengdu, Sichuan 610065, China.
Shanghai Key Laboratory of Orthopaedic Surgery of Shanghai Ninth People's Hospital, Shanghai Jiao Tong University, Shanghai 200011, China; School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200030, China.
Colloids Surf B Biointerfaces. 2025 Mar;247:114397. doi: 10.1016/j.colsurfb.2024.114397. Epub 2024 Nov 22.
Instigating oxidative stress is a crucial aspect of antibacterial therapy. Yet, its behavior is poorly understood in the context of zeolitic imidazolate frameworks (ZIFs) - a group of highly promising antibacterial agents. To address this gap, a series of ZIF@Ce6 particles were synthesized to investigate the impact of particle shape, size, and metal ion type on oxidative stress and bactericidal activity. For the first time, the interplay between the physicochemical properties and antibacterial activities of different ZIF@Ce6 particles is demonstrated, while unearthing their oxidative stress strategy in photodynamic therapy. Notably, the incorporation of chlorin e6 (Ce6), combined with light irradiation, amplified the bactericidal effect of the ZIFs and achieved a rare minimum inhibition concentration (MIC) of 12.5 µgmL for ZIF-8. We discovered that singlet oxygen (O) production varies with particle shape and size, while photodynamic activity reshuffles the antibacterial performance sequence from pristine to modified ZIF-8. Interestingly, reactive oxygen species (ROS) accumulation and glutathione depletion tests revealed that oxidative stress in pristine ZIF-8 is predominantly induced by ROS, whereas both ROS and glutathione contribute to the oxidative stress in ZIF@Ce6 and pristine ZIF-67. When bacteria are preincubated with the antioxidant N-acetyl cysteine, the bactericidal activity of ZIF@Ce6 increases while the activity of pristine ZIF-8 is reduced and that of ZIF-67 remains unchanged. This study deepens our understanding of the antibacterial properties of ZIFs and their oxidative stress paths, paving way for the fabrication of ZIF-based materials with enriched and targeted antibacterial properties.
引发氧化应激是抗菌治疗的一个关键方面。然而，在沸石咪唑酯骨架（ZIFs）——一类极具前景的抗菌剂——的背景下，其行为却鲜为人知。为了填补这一空白，合成了一系列ZIF@Ce6颗粒，以研究颗粒形状、尺寸和金属离子类型对氧化应激和杀菌活性的影响。首次证明了不同ZIF@Ce6颗粒的物理化学性质与抗菌活性之间的相互作用，同时揭示了它们在光动力疗法中的氧化应激策略。值得注意的是，引入二氢卟吩e6（Ce6）并结合光照，放大了ZIFs的杀菌效果，并使ZIF-8达到了罕见的12.5 µg/mL的最低抑菌浓度（MIC）。我们发现单线态氧（O）的产生随颗粒形状和尺寸而变化，而光动力活性改变了从原始ZIF-8到改性ZIF-8的抗菌性能顺序。有趣的是，活性氧（ROS）积累和谷胱甘肽消耗测试表明，原始ZIF-8中的氧化应激主要由ROS诱导，而ROS和谷胱甘肽都对ZIF@Ce6和原始ZIF-67中的氧化应激有贡献。当细菌与抗氧化剂N-乙酰半胱氨酸预孵育时，ZIF@Ce6的杀菌活性增加，而原始ZIF-8的活性降低，ZIF-67的活性保持不变。这项研究加深了我们对ZIFs抗菌特性及其氧化应激途径的理解，为制备具有丰富和靶向抗菌特性的ZIF基材料铺平了道路。
