Department of Otorhinolaryngology, Head and Neck Surgery, Section of Experimental Oncology and Nanomedicine (SEON), Else Kröner-Fresenius-Stiftung Professorship, Universitätsklinikum Erlangen, Erlangen, Germany.
Mikrobiologisches Institut - Klinische Mikrobiologie, Immunologie und Hygiene, Universitätsklinikum Erlangen, Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg, Erlangen, Germany.
Int J Nanomedicine. 2023 Jun 14;18:3231-3246. doi: 10.2147/IJN.S409559. eCollection 2023.
Magnetic separation of microbes can be an effective tool for pathogen identification and diagnostic applications to reduce the time needed for sample preparation. After peptide functionalization of superparamagnetic iron oxide nanoparticles (SPIONs) with an appropriate interface, they can be used for the separation of sepsis-associated yeasts like . Due to their magnetic properties, the magnetic extraction of the particles in the presence of an external magnetic field ensures the accumulation of the targeted yeast.
In this study, we used SPIONs coated with 3-aminopropyltriethoxysilane (APTES) and functionalized with a peptide originating from GP340 (SPION-APTES-Pep). For the first time, we investigate whether this system is suitable for the separation and enrichment of , we investigated its physicochemical properties and by thermogravimetric analysis we determined the amount of peptide on the SPIONs. Further, the toxicological profile was evaluated by recording cell cycle and DNA degradation. The separation efficiency was investigated using in different experimental settings, and regrowth experiments were carried out to show the use of SPION-APTES-Pep as a sample preparation method for the identification of fungal infections.
SPION-APTES-Pep can magnetically remove more than 80% of the microorganism and with a high selective host-pathogen distinction from water-based media and about 55% in blood after 8 minutes processing without compromising effects on the cell cycle of human blood cells. Moreover, the separated fungal cells could be regrown without any restrictions.
微生物的磁分离可以成为一种用于病原体鉴定和诊断应用的有效工具，以减少样品制备所需的时间。在超顺磁氧化铁纳米粒子（SPION）上进行适当的界面肽功能化后，它们可以用于分离脓毒症相关的酵母，如 。由于其磁性，在外磁场存在下，颗粒的磁性提取可确保目标酵母的聚集。
在本研究中，我们使用了涂有 3-氨丙基三乙氧基硅烷（APTES）并用来自 GP340 的肽功能化的 SPION（SPION-APTES-Pep）。我们首次研究了该系统是否适合分离和富集 ，我们研究了其物理化学性质，并通过热重分析确定了 SPION 上肽的量。此外，通过记录细胞周期和 DNA 降解来评估毒理学特征。使用 在不同的实验设置中研究了分离效率，并进行了再生实验以显示 SPION-APTES-Pep 作为用于鉴定真菌感染的样品制备方法的用途。
SPION-APTES-Pep 可以从基于水的介质中磁性去除超过 80%的微生物，并且具有高选择性的宿主-病原体区分 ，在 8 分钟的处理后在血液中的去除率约为 55%，而不会对人血红细胞的细胞周期产生不利影响。此外，分离的真菌细胞可以不受任何限制地再生。
