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开发玉米象高密度连锁图谱的研究进展。

Development of the first high-density linkage map in the maize weevil, .

机构信息

Genetic Engineering and Society Center, North Carolina State University, Raleigh, NC, United States.

Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, North Carolina, United States.

出版信息

PeerJ. 2023 Jun 15;11:e15414. doi: 10.7717/peerj.15414. eCollection 2023.

Abstract

The maize weevil, , is a worldwide pest that disproportionately affects subsistence farmers in developing countries. Damage from this pest threatens food security in these communities as widely available and effective control methods are lacking. With advances over the last decade in the development of genetic pest management techniques, addressing pest issues at the ecosystem level as opposed to the farm level may be a possibility. However, pest species selected for genetic management techniques require a well-characterized genome and few genomic tools have been developed for . Here, we have measured the genome size and developed the first genetic linkage map for this species. The genome size was determined using flow cytometry as 682 Mb and 674 Mb for females and males, respectively. The linkage map contains 11 linkage groups, which correspond to the 10 autosomes and 1 X-chromosome found in the species and it contains 1,121 SNPs. This linkage map will be useful for assembling a complete genome for .

摘要

玉米象, 是一种世界性的害虫,它对发展中国家的自给农民造成了不成比例的影响。由于缺乏广泛可用且有效的控制方法,这种害虫的破坏威胁着这些社区的粮食安全。在过去十年中,遗传害虫管理技术的发展取得了进展,因此可能可以在生态系统层面而不是在农场层面解决害虫问题。然而,用于遗传管理技术的害虫物种需要具有特征明确的基因组,而针对 开发的基因组工具却很少。在这里,我们测量了该物种的基因组大小并开发了第一个遗传连锁图谱。使用流式细胞术分别确定雌性和雄性的基因组大小为 682 Mb 和 674 Mb。连锁图谱包含 11 个连锁群,分别对应于该物种的 10 条常染色体和 1 条 X 染色体,其中包含 1121 个 SNP。该连锁图谱将有助于为 组装完整的基因组。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9da2/10276983/a565afebb227/peerj-11-15414-g001.jpg

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