Woo S L, Orlando C A, Camp J F, Akeson W H
J Biomech. 1986;19(5):399-404. doi: 10.1016/0021-9290(86)90016-3.
The purpose of this study is to examine the effect of prolonged postmortem freezing storage (between 1 1/2 and 3 months at -20 degrees C) on the structural properties of the medial collateral ligament (MCL)-bone complex as well as the mechanical properties of the MCL substance from the rabbit knee. Tensile testing of the femur-MCL-tibia specimen was performed and no statistically significant changes were noted between the fresh and stored samples in terms of the cyclic stress relaxation, the load-deformation characteristics, as well as the load, deformation and energy absorbing capability at failure. The area of hysteresis of the stored samples was significantly reduced in the first few cycles, however. The mechanical properties of the MCL substance, as represented by the stress-strain curves, tensile strength and ultimate strain also did not change following storage. We conclude, therefore, proper and careful storage by freezing would have little or no effect on the biomechanical properties of the ligaments.
本研究的目的是检验长时间死后冷冻保存（在-20℃下保存1个半月至3个月）对兔膝关节内侧副韧带（MCL）-骨复合体结构特性以及MCL组织力学特性的影响。对股骨-MCL-胫骨标本进行了拉伸试验，新鲜样本与保存样本在循环应力松弛、载荷-变形特性以及失效时的载荷、变形和能量吸收能力方面均未观察到统计学上的显著变化。不过，保存样本的滞后面积在最初几个循环中显著减小。以应力-应变曲线、拉伸强度和极限应变表示的MCL组织力学特性在保存后也未发生变化。因此，我们得出结论，适当且小心的冷冻保存对韧带的生物力学特性几乎没有影响。
