Xu Yong-Nan, Han Guo-Bo, Li Ying-Hua, Piao Chun-Hao, Li Guan-Hao, Kim Nam-Hyung
School of Biotechnology and Health Sciences, Wuyi University, Jiangmen, 529000, China.
Jilin Wangqing Animal Quarantine Station, Wangqing, 133200, China.
Zygote. 2023 Oct;31(5):451-456. doi: 10.1017/S0967199423000199. Epub 2023 Jun 20.
Mammalian oocytes not fertilized immediately after ovulation can undergo ageing and a rapid decline in quality. The addition of antioxidants can be an efficient approach to delaying the oocyte ageing process. Onion peel extract (OPE) contains quercetin and other flavonoids with natural antioxidant activities. In this study, we investigated the effect of OPE on mouse oocyte ageing and its mechanism of action. The oocytes were aged in M16 medium for 16 h after adding OPE at different concentrations (0, 50, 100, 200, and 500 μg/ml). The addition of 100 μg/ml OPE reduced the oocyte fragmentation rate, decreased the reactive oxygen species (ROS) level, increased the glutathione (GSH) level, and improved the mitochondrial membrane potential compared with the control group. The addition of OPE also increased the expression of , , and genes, and the caspase-3 activity in OPE-treated aged oocytes was significantly lower than that in untreated aged oocytes and similar to that in fresh oocytes. These results indicated that OPE delayed mouse oocyte ageing by reducing oxidative stress and apoptosis and enhancing mitochondrial function.
排卵后未立即受精的哺乳动物卵母细胞会发生老化，质量迅速下降。添加抗氧化剂可能是延缓卵母细胞老化过程的有效方法。洋葱皮提取物（OPE）含有槲皮素和其他具有天然抗氧化活性的黄酮类化合物。在本研究中，我们研究了OPE对小鼠卵母细胞老化的影响及其作用机制。在添加不同浓度（0、50、100、200和500μg/ml）的OPE后，将卵母细胞在M16培养基中老化16小时。与对照组相比，添加100μg/ml OPE可降低卵母细胞碎片率，降低活性氧（ROS）水平，提高谷胱甘肽（GSH）水平，并改善线粒体膜电位。添加OPE还增加了 、 和 基因的表达，且经OPE处理的老化卵母细胞中的半胱天冬酶-3活性显著低于未处理的老化卵母细胞，与新鲜卵母细胞中的活性相似。这些结果表明，OPE通过降低氧化应激和细胞凋亡并增强线粒体功能来延缓小鼠卵母细胞老化。
