Jeon Hyuk-Joon, You Seung Yeop, Kim Dong Hyun, Jeon Hong Bae, Oh Jeong Su
Department of Genetic Engineering,College of Biotechnology and Bioengineering,Sungkyunkwan University,Suwon,Korea.
Biomedical Research Institute,MEDIPOST Co.,Ltd.,Seongnam,Korea.
Zygote. 2017 Aug;25(4):472-479. doi: 10.1017/S0967199417000296. Epub 2017 Jun 21.
Following ovulation, oocytes undergo a time-dependent deterioration in quality referred to as post-ovulatory ageing. Although various factors influence the post-ovulatory ageing of oocytes, oxidative stress is a key factor involved in deterioration of oocyte quality. Artemisia asiatica Nakai ex Pamp. has been widely used in East Asia as a food ingredient and traditional medicine for the treatment of inflammation, cancer, and microbial infections. Recent studies have shown that A. asiatica exhibits antioxidative effects. In this study, we investigated whether A. asiatica has the potential to attenuate deterioration in oocyte quality during post-ovulatory ageing. Freshly ovulated mouse oocytes were cultured with 0, 50, 100 or 200 μg/ml ethanol extracts of A. asiatica Nakai ex Pamp. After culture for up to 24 h, various ageing-induced oocyte abnormalities, including morphological changes, reactive oxygen species (ROS) accumulation, apoptosis, chromosome and spindle defects, and mitochondrial aggregation were determined. Treatment of oocytes with A. asiatica extracts reduced ageing-induced morphological changes. Moreover, A. asiatica extracts decreased ROS generation and the onset of apoptosis by preventing elevation of the Bax/Bcl-2 expression ratio during post-ovulatory ageing. Furthermore, A. asiatica extracts attenuated the ageing-induced abnormalities including spindle defects, chromosome misalignment and mitochondrial aggregation. Our results demonstrate that A. asiatica can relieve deterioration in oocyte quality and delay the onset of apoptosis during post-ovulatory ageing.
排卵后，卵母细胞会经历质量随时间下降的过程，即排卵后老化。尽管多种因素会影响卵母细胞的排卵后老化，但氧化应激是导致卵母细胞质量下降的关键因素。东亚唐松草已在东亚广泛用作食品成分和传统药物，用于治疗炎症、癌症和微生物感染。最近的研究表明，东亚唐松草具有抗氧化作用。在本研究中，我们调查了东亚唐松草是否有潜力减轻排卵后老化过程中卵母细胞质量的下降。将刚排卵的小鼠卵母细胞与0、50、100或200μg/ml东亚唐松草乙醇提取物一起培养。培养长达24小时后，确定各种老化诱导的卵母细胞异常，包括形态变化、活性氧（ROS）积累、凋亡、染色体和纺锤体缺陷以及线粒体聚集。用东亚唐松草提取物处理卵母细胞可减少老化诱导的形态变化。此外，东亚唐松草提取物通过防止排卵后老化过程中Bax/Bcl-2表达比值升高，减少了ROS的产生和凋亡的发生。此外，东亚唐松草提取物减轻了老化诱导的异常，包括纺锤体缺陷、染色体排列紊乱和线粒体聚集。我们的结果表明，东亚唐松草可以缓解卵母细胞质量的下降，并延迟排卵后老化过程中凋亡的发生。
