Guangzhou Red Cross Hospital, Guangzhou, China.
School of Forensic Medicine, Southern Medical University, Guangzhou, China.
Ann Hum Genet. 2023 Sep;87(5):232-240. doi: 10.1111/ahg.12518. Epub 2023 Jun 20.
X chromosomeshort tandem repeat (X-STR) loci are playing an increasingly important role inforensic work, identifying female traces in male contamination and explainingcomplex kinship analyses.
In this study, we analyzed thegenetic polymorphism of 19 X-STR loci in the Guangdong Hakka, Teochew and Cantonese groups, respectively, aswell as in the Guangdong Hakka, Teochew andCantonese pooled Han. The genetic diversity and forensic characteristics of the19 X-STRs and 7 linkage groups were investigated, respectively.
The experiments showed that the genetic diversity (GD) and polymorphism information content (PIC) in the pooledGuangdong Han ranged from 0.5320 to 0.9234 and 0.4369 to 0.9171, respectively, and the cumulative power of discrimination for males (PDM), power of discrimination for females (PDF) and mean paternity exclusion chance (MEC) were higher than 0.9999999, indicating that the 19 X-STRs had high geneticpolymorphism and discriminatory power. Genetic differences among Chinese Hansubgroups and among different Chinese populations were investigated byphylogenetic reconstruction and principal component analysis (PCA), respectively. Genetic analyses based on neighbor-joining (NJ) tree and principal component analysis plot showed that Cantonese, Teochew and Hakka were closely genetically related, and different populations with closer linguistic components had more genetic affinity.
This study adds to the forensic X-STR database and demonstrates the forensic efficiency of 19 X-STRs for the Hakka, Teochewand Cantonese populations in Guangdong, and the pooled Han of Hakka, Teochewand Cantonese people in Guangdong.
X 染色体短串联重复（X-STR）基因座在法医工作中发挥着越来越重要的作用，可用于鉴定男性污染样本中的女性痕迹，以及解释复杂的亲缘关系分析。
本研究分析了广东客家、潮汕和广府群体，以及广东客家、潮汕和广府汉族人群中 19 个 X-STR 基因座的遗传多态性。分别研究了 19 个 X-STR 及 7 个连锁群的遗传多样性和法医学特征。
实验表明，广东汉族混合群体的遗传多样性（GD）和多态信息含量（PIC）分别为 0.5320-0.9234 和 0.4369-0.9171，男性个体鉴别力（PDM）、女性个体鉴别力（PDF）和平均父权排除概率（MEC）均大于 0.9999999，表明 19 个 X-STR 具有较高的遗传多态性和鉴别能力。通过系统发生重建和主成分分析（PCA）分别研究了中国汉族亚群之间和不同中国人群之间的遗传差异。基于邻接（NJ）树和主成分分析图的遗传分析表明，广府、潮汕和客家在遗传上密切相关，具有更密切语言成分的不同人群具有更高的遗传亲和力。
本研究为广东客家、潮汕和广府人群的法医 X-STR 数据库增添了新内容，验证了 19 个 X-STR 对广东客家、潮汕和广府人群及广东客家、潮汕和广府汉族人群的法医效率。
