血清吲哚硫酸酯和对甲酚在伴有和不伴有肾病的 2 型糖尿病患者中的水平。

Serum Levels of Indoxyl Sulfate and P-cresol in Type II Diabetic Patients With and Without Nephropathy.

机构信息

Pharmaceutical Analysis Research Center and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.

出版信息

Iran J Kidney Dis. 2023 May;17(3):126-134.

Abstract

INTRODUCTION

Indoxyl sulfate (IS) and para-cresol (p-cresol) are uremic toxins with high protein bonding index that accumulate in the body with decreasing kidney function. The main purpose of the current investigation was to compare the concentration of p-cresol and IS in serum of the type II diabetic individuals with and without nephropathy.

METHODS

Fifty-five patients with type II diabetes mellitus were divided into two groups: case and control. The case group consisted of 26 diabetic patients with nephropathy (proteinuria and serum creatinine below 1.5 mg/dL) without any other kidney diseases. The control group included 29 patients without diabetic nephropathy. Patients with advanced heart disease, cerebrovascular accident and other inflammatory or infectious diseases were excluded. Five mL of venous blood was taken from each patient in the morning fasting state. Then other laboratory tests including serum uric acid and creatinine levels, serum urea nitrogen, lipids and glucose were measured by standard methods. P-Cresol and IS levels were measured by the spectrofluorimetric method after extraction. We also filled out a checklist with information regarding the duration of their disease, medication history (oral or injectable), and other demographic information. There were no significant differences between the two groups regarding the investigated factors Results. There were no significant difference among the investigated factors between the two groups (P > .05) except for the serum creatinine, proteinuria and estimated glomerular filtration rate, where the mean values of cases were considerably higher than those of the controls. Serum IS and p-cresol levels were also significantly higher in the case group (P < .05).

CONCLUSION

According to the findings, it seems that IS, and p-cresol may play a role in the development of diabetic nephropathy and other complications of diabetes mellitus. DOI: 10.52547/ijkd.7266.

摘要

简介

硫酸吲哚酚（IS）和对甲酚（p-cresol）是与蛋白质结合率高的尿毒症毒素，随着肾功能的下降而在体内蓄积。本研究的主要目的是比较伴有和不伴有肾病的 2 型糖尿病患者血清中 p-cresol 和 IS 的浓度。

方法

将 55 例 2 型糖尿病患者分为两组：病例组和对照组。病例组由 26 例伴有肾病（蛋白尿和血清肌酐低于 1.5mg/dL）的糖尿病患者组成，无其他肾脏疾病。对照组包括 29 例无糖尿病肾病的患者。排除晚期心脏病、脑血管意外和其他炎症或感染性疾病的患者。每个患者在空腹状态下抽取 5mL 静脉血。然后采用标准方法测量其他实验室检查包括血清尿酸和肌酐水平、血清尿素氮、血脂和血糖。通过提取后用荧光分光光度法测量 p-cresol 和 IS 水平。我们还填写了一份检查表，内容包括疾病持续时间、用药史（口服或注射）和其他人口统计学信息。两组间调查因素结果无显著差异。两组间除血清肌酐、蛋白尿和估计肾小球滤过率外，其他调查因素无显著差异，病例组均值明显高于对照组。病例组血清 IS 和 p-cresol 水平也显著升高（P <.05）。