Department of Biochemistry, Faculty of Veterinary Medicine, Balikesir University, Balikesir, Turkey.
Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Balikesir University, Balikesir, Turkey.
Biotech Histochem. 2023 Nov;98(6):432-444. doi: 10.1080/10520295.2023.2224061. Epub 2023 Jun 20.
Ulcerative colitis (UC) is an inflammatory bowel disease of unknown etiology for which effective treatments are lacking. Scutellarin is a flavonoid with anti-apoptotic, antioxidant and anti-inflammatory effects. We investigated the possible protective effects of scutellarin on UC induced by acetic acid in rats. We used five groups of male rats: control, scutellarin, UC, UC + scutellarin, UC + sulfasalazine. Colonic mucosal inflammation was evaluated microscopically. Malondialdehyde (MDA), superoxide dismutase (SOD) activity, total antioxidant status (TAS), nitric oxide (NO), interleukin 6 (IL-6), tumor necrosis factor-α (TNF-α) and DNA fragmentation levels were measured. Colon tissue sections were evaluated using immunohistochemistry for Bcl-2 and Bax, TUNEL staining and histopathology. Pretreatment with scutellarin significantly reduced histological damage. Scutellarin significantly reduced serum and tissue levels of MDA, NO, IL-6 and TNF-α, and increased enzymatic activity of SOD and TAS. Scutellarin suppressed apoptosis by down-regulation of Bax, reduction of DNA fragmentation and increased expression of Bcl-2. Apoptosis was increased, while antioxidant enzymes were significantly decreased; some histopathologic disorders also were seen in the UC group. Administration of scutellarin ameliorated the pathological and biochemical alterations caused by UC in rats. We found that scutellarin might be protective against UC by down-regulating pro-inflammatory cytokines and suppressing apoptosis and oxidative stress.
溃疡性结肠炎（UC）是一种病因不明的炎症性肠病，缺乏有效治疗方法。野黄芩苷是一种具有抗凋亡、抗氧化和抗炎作用的黄酮类化合物。我们研究了野黄芩苷对乙酸诱导的大鼠 UC 的可能保护作用。我们使用了五组雄性大鼠：对照组、野黄芩苷组、UC 组、UC+野黄芩苷组、UC+柳氮磺胺吡啶组。通过显微镜评估结肠黏膜炎症。测量丙二醛（MDA）、超氧化物歧化酶（SOD）活性、总抗氧化状态（TAS）、一氧化氮（NO）、白细胞介素 6（IL-6）、肿瘤坏死因子-α（TNF-α）和 DNA 片段化水平。使用免疫组织化学法评估结肠组织切片中 Bcl-2 和 Bax、TUNEL 染色和组织病理学。野黄芩苷预处理可显著减轻组织学损伤。野黄芩苷可显著降低血清和组织中 MDA、NO、IL-6 和 TNF-α的水平，并增加 SOD 和 TAS 的酶活性。野黄芩苷通过下调 Bax、减少 DNA 片段化和增加 Bcl-2 表达来抑制细胞凋亡。凋亡增加，而抗氧化酶显著减少；UC 组也出现了一些组织病理学紊乱。给予野黄芩苷可改善 UC 大鼠的病理和生化改变。我们发现，野黄芩苷可能通过下调促炎细胞因子和抑制细胞凋亡和氧化应激来保护 UC。
