Demirel Metin, Köktaşoğlu Fatmanur, Özkan Esin, Dulun Ağaç Halime, Gül Ayşe Zehra, Sharifov Rasul, Sarıkaya Ufuk, Başaranoğlu Metin, Selek Şahabettin
Department of Medical Biochemistry, Bezmialem Vakif University Faculty of Medicine, Istanbul, Turkey.
Health Sciences Institute, Bezmialem Vakif University, Istanbul, Turkey.
Scand J Gastroenterol. 2023 Jul-Dec;58(11):1344-1350. doi: 10.1080/00365521.2023.2225667. Epub 2023 Jun 20.
Non-alcoholic fatty liver disease (NAFLD) is a disease characterized by the accumulation of excessive fat in the liver, which can lead to fibrosis and has an increasing prevalence. NAFLD requires non-invasive diagnostic biomarkers. While typically observed in overweight individuals, it can also occur in non-obese/non-overweight individuals. Comparative studies on non-obese NAFLD patients are scarce. This study aimed to conduct a using liquid chromatography-high resolution mass spectrometry (LC-MS/MS)-based metabolic profiling of non-obese NAFLD patients and healthy controls.
The patient group consisted of 27 individuals with NAFLD, while the healthy control group included 39 individuals. Both groups were between 18 and 40 years old, had a BMI of less than 25 and had alcohol consumption less than 20 g/week for men and 10 g/week for women. Serum samples were collected and analyzed using LC-MS/MS. The data were analyzed using the TidyMass and MetaboAnalyst.
The LC-MS/MS analyses detected significant changes in D-amino acid metabolism, vitamin B6 metabolism, apoptosis, mTOR signaling pathway, lysine degradation, and phenylalanine metabolism pathways in non-obese NAFLD patients. Significant changes were also observed in the metabolites D-pantothenic acid, hypoxanthine, citric acid, citramalic acid, L-phenylalanine, glutamine, and histamine-trifluoromethyl-toluidide, β-hydroxymyristic acid, DL-Lactic acid, and 3-methyl-2-oxopentanoic. Overall, the study provides valuable insights into the metabolic changes associated with non-obese NAFLD patients and can contribute to the development of non-invasive diagnostic biomarkers for NAFLD.
This study sheds light on the metabolic changes in non-obese NAFLD patients. Further research is needed to better understand the metabolic changes associated with NAFLD and to develop effective treatment options.
非酒精性脂肪性肝病（NAFLD）是一种以肝脏中过量脂肪堆积为特征的疾病，可导致肝纤维化，且患病率不断上升。NAFLD需要非侵入性诊断生物标志物。虽然通常在超重个体中观察到，但它也可能发生在非肥胖/非超重个体中。关于非肥胖NAFLD患者的比较研究很少。本研究旨在对非肥胖NAFLD患者和健康对照者进行基于液相色谱-高分辨率质谱（LC-MS/MS）的代谢谱分析。
患者组由27例NAFLD患者组成，健康对照组包括39例个体。两组年龄均在18至40岁之间，BMI小于25，男性每周饮酒量小于20克，女性每周饮酒量小于10克。采集血清样本并使用LC-MS/MS进行分析。数据使用TidyMass和MetaboAnalyst进行分析。
LC-MS/MS分析检测到非肥胖NAFLD患者在D-氨基酸代谢、维生素B6代谢、细胞凋亡、mTOR信号通路、赖氨酸降解和苯丙氨酸代谢途径中有显著变化。在代谢物D-泛酸、次黄嘌呤、柠檬酸、柠苹酸、L-苯丙氨酸、谷氨酰胺、组胺-三氟甲基-甲苯胺、β-羟基肉豆蔻酸、DL-乳酸和3-甲基-2-氧代戊酸中也观察到显著变化。总体而言，该研究为与非肥胖NAFLD患者相关的代谢变化提供了有价值的见解，并有助于开发NAFLD的非侵入性诊断生物标志物。
本研究揭示了非肥胖NAFLD患者代谢变化情况。需要进一步研究以更好地理解与NAFLD相关的代谢变化并开发有效的治疗方案。
