National Key Discipline Laboratory, Department of Nutrition and Food Hygiene, School of Public Health, Harbin Medical University, Harbin, PR China.
National Key Discipline Laboratory, Department of Nutrition and Food Hygiene, School of Public Health, Harbin Medical University, Harbin, PR China.
J Pharm Biomed Anal. 2021 Jun 5;200:114058. doi: 10.1016/j.jpba.2021.114058. Epub 2021 Apr 1.
The aim of this study was to identify novel serum metabolites associated with non-alcoholic fatty liver disease (NAFLD), and to explore the metabolic discrepancies between Lean-NAFLD and Obese-NAFLD.
Serum samples from patients with NAFLD (n = 161) and healthy participants (n = 149) were collected, and metabolites were analyzed with UPLC-Q-TOF MS/MS. Subgroup analyses were performed to explore the metabolic differences among Lean-NAFLD, Obese-NAFLD and healthy controls RESULTS: A total of 24 differentially present metabolites were found between patients with NAFLD and healthy controls. Marked metabolic pathway differences were observed among the NAFLD subtypes, including in fatty acid and amino acid metabolism. Ultimately, five metabolites (prasterone, indoxylsulfuric acid, sebacic acid, arachidonic acid and pregnenolone sulfate) were used to establish a diagnostic model to distinguish patients with NAFLD regardless of Lean- or Obese-NAFLD type.
This study suggested that significant metabolic differences existed among subtypes of NAFLD, and our model might be useful to distinguish patients with NAFLD. These findings may lay a foundation for the detection and treatment of NAFLD subtypes.
本研究旨在鉴定与非酒精性脂肪性肝病（NAFLD）相关的新型血清代谢物，并探讨瘦型 NAFLD 和肥胖型 NAFLD 之间的代谢差异。
收集 NAFLD 患者（n=161）和健康对照者（n=149）的血清样本，采用 UPLC-Q-TOF MS/MS 分析代谢物。进行亚组分析以探讨瘦型 NAFLD、肥胖型 NAFLD 和健康对照组之间的代谢差异。
在 NAFLD 患者和健康对照者之间共发现 24 种差异表达的代谢物。在 NAFLD 亚型之间观察到明显的代谢途径差异，包括脂肪酸和氨基酸代谢。最终，使用 5 种代谢物（孕烯醇酮、吲哚硫酸、癸二酸、花生四烯酸和孕烷醇酮硫酸盐）建立了一个诊断模型，可用于区分无论瘦型或肥胖型 NAFLD 类型的患者。
本研究表明，NAFLD 亚型之间存在显著的代谢差异，我们的模型可能有助于区分 NAFLD 患者。这些发现可能为 NAFLD 亚型的检测和治疗奠定基础。
