De Greve J, van der Elst J, Willems G
J Cancer Res Clin Oncol. 1986;111(3):191-5. doi: 10.1007/BF00389233.
Colon carcinoma was implanted into the mesenterium of syngeneic BD IX rats, and 10 weeks later the animals were fasted for 48 h and refed. Control animals were kept fasted for an additional 24-h period. DNA synthesis was measured in mucosal scrapings from the normal colon and in the tumor before and after refeeding. Autoradiography was used to determine the epithelial DNA synthesis index (labeling index) in the progenitor cells from normal colon mucosa, in the adenocarcinoma cells from the tumor, and in the tumor lymphocytes. DNA synthesis increased over control values in the normal mucosa in situ (p less than 0.01), and in the implanted tumor (p less than 0.05) at 16 h after refeeding. The labeling index also increased over control values in the progenitor cells from normal mucosa (p less than 0.01), and in the adenocarcinoma cells from the tumor (p less than 0.01) at 16 h after refeeding. No increase in labeling index was observed in the tumor lymphocytes. These data suggest that cell proliferation in normal colon epithelium as well as in colon adenocarcinoma cells may be stimulated by a common physiological factor released after feeding.
将结肠癌植入同基因BD IX大鼠的肠系膜中，10周后让动物禁食48小时，然后再喂食。对照动物再额外禁食24小时。在再喂食前后，测量正常结肠黏膜刮片以及肿瘤中的DNA合成。利用放射自显影术来确定正常结肠黏膜祖细胞、肿瘤腺癌细胞以及肿瘤淋巴细胞中的上皮DNA合成指数（标记指数）。再喂食16小时后，正常原位黏膜（p<0.01）和植入肿瘤（p<0.05）中的DNA合成高于对照值。再喂食16小时后，正常黏膜祖细胞（p<0.01）和肿瘤腺癌细胞（p<0.01）中的标记指数也高于对照值。肿瘤淋巴细胞中未观察到标记指数增加。这些数据表明，喂食后释放的一种共同生理因素可能刺激正常结肠上皮以及结肠腺癌细胞中的细胞增殖。
