microRNAs 与膀胱癌的关系：microRNAs 是否有助于预测疑似膀胱癌患者的膀胱癌？
The relationship between microRNAs and bladder cancer: are microRNAs useful to predict bladder cancer in suspicious patients?
机构信息
Department of Urology, Private Yuzyil Gebze Hospital, Sultan Orhan Mahallesi, Ilyasbey Cd. No:38, 41400, Gebze, Kocaeli, Turkey.
Department of Urology, Fırat University Medical Faculty, Elazıg, Turkey.
出版信息
Int Urol Nephrol. 2023 Oct;55(10):2483-2491. doi: 10.1007/s11255-023-03666-2. Epub 2023 Jun 20.
PURPOSE
Recent studies indicate that circulating micro RNAs (miRNAs) are novel class of non-invasive biomarkers with diagnostic and prognostic information. We evaluated the miRNA expressions in bladder cancer (BC) and their associations with disease diagnosis.
METHODS
We profiled the expressions of 379 miRNAs in the plasma samples from patients with non-muscle invasive bladder cancer (NMIBC) (n = 34) and non-malignant urological diseases as a control group (n = 32). Patients were evaluated regarding with age, miRNA expressions, by using descriptive statistics. miRNA expression in extracted RNA was quantified using the NanoString nCounter Digital Analyzer.
RESULTS
The analysis of plasma miRNA levels in the marker identification cohort indicated that plasma (miR-1260a, let-7a-3p miR-196b-5p, miR-196a-5p, miR-99a-5p, miR-615-5p, miR-4301, miR-28-3p, miR-4538, miR-1233-3p, miR-4732-5p, miR-1913, miR-1280) levels were increased in NMIBC patients compared to control subjects. There were no significant differences other parameters studied between groups.
CONCLUSIONS
The analysis of serum plasma miRNA (miR-1260a, let-7a-3p miR-196b-5p, miR-196a-5p, miR-99a-5p, miR-615-5p, miR-4301, miR-28-3p, miR-4538, miR-1233-3p, miR-4732-5p, miR-1913, miR-1280) levels could be useful plasma biomarkers for BC.
目的
最近的研究表明，循环 microRNAs（miRNAs）是一类新型的非侵入性生物标志物，具有诊断和预后信息。我们评估了膀胱癌（BC）中的 miRNA 表达及其与疾病诊断的关联。
方法
我们在非肌肉浸润性膀胱癌（NMIBC）患者（n=34）和非恶性泌尿科疾病患者（n=32）的血浆样本中分析了 379 种 miRNA 的表达。使用描述性统计对患者的年龄和 miRNA 表达进行评估。使用 NanoString nCounter Digital Analyzer 定量提取 RNA 中的 miRNA 表达。
结果
在标志物识别队列中分析血浆 miRNA 水平表明，与对照组相比，NMIBC 患者的血浆（miR-1260a、let-7a-3p、miR-196b-5p、miR-196a-5p、miR-99a-5p、miR-615-5p、miR-4301、miR-28-3p、miR-4538、miR-1233-3p、miR-4732-5p、miR-1913、miR-1280）水平升高。两组之间其他研究参数无显著差异。
结论
血清血浆 miRNA（miR-1260a、let-7a-3p、miR-196b-5p、miR-196a-5p、miR-99a-5p、miR-615-5p、miR-4301、miR-28-3p、miR-4538、miR-1233-3p、miR-4732-5p、miR-1913、miR-1280）水平分析可能成为 BC 的有用血浆生物标志物。
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