Krajewski S, Rathmer K, Hamada H, Weizsäcker M, Eberhardt B, Wechsler W
J Cancer Res Clin Oncol. 1986;112(1):33-8. doi: 10.1007/BF00394936.
The subcapsular renal transplantation tumor model was explored to standardize the growth of rat RN6 neurinoma and RG2 glioma nitrosourea-induced clonal cell lines in syngeneic and allogeneic systems. Growth of RN6 and RG2 tumor spheroids was compared with that of solid subcutaneous tumor pieces transplanted under the renal capsule. Two morphometrical methods were applied to evaluate growth rates. Tumor specimens were examined histologically with regard to their morphology, extent of immune reactions, and development of tumor necroses. The take rate was 98%. In the syngeneic system linear progressive tumor growth was found, while in preirradiated allogeneic rats this was only the case up to 21 to 25 days post transplantation (p.t.). Strong rejection reactions in the allogeneic RN6 tumors were noted from 4 to 7 days p.t. resulting in total tumor rejection after 10 to 14 days. Both kinds of tumors, especially in the first days of growth, were characterized by strong desmoplastic reaction with rich reticulin fiber formation. However, after 10 days, in the center of RG2 subcapsular renal tumors (SRT) this kind of reaction was found only in the vicinity of tumor vessels, while RN6 SRT demonstrated reticulin fibers around tumor cells in all cases studied. The transplantation experiments revealed that the malignant RN6 and RG2 spheroids represent a suitable tool to study three-dimensional early tumor growth in both in vivo and in vitro cultures. The model of spheroid transplantation under the renal capsule is simple to handle and well reproducible. Compared with subcutaneous tumors the SRT model has advantages in early stages of tumor growth because the tumors are clearly visible grossly and can be easily submitted to adequate morphometry, indicating that this model may be suitable for experimental chemotherapy and radiotherapy studies.
探索了肾包膜下肾移植肿瘤模型，以规范大鼠RN6神经鞘瘤和RG2胶质瘤亚硝基脲诱导的克隆细胞系在同基因和异基因系统中的生长。将RN6和RG2肿瘤球体的生长与肾包膜下移植的实体皮下肿瘤块的生长进行了比较。应用两种形态计量学方法评估生长速率。对肿瘤标本进行组织学检查，观察其形态、免疫反应程度和肿瘤坏死情况。接种率为98%。在同基因系统中发现肿瘤呈线性渐进性生长，而在预先照射的异基因大鼠中，仅在移植后21至25天内如此。在异基因RN6肿瘤中，在移植后4至7天观察到强烈的排斥反应，导致在10至14天后肿瘤完全被排斥。两种肿瘤，尤其是在生长的最初几天，其特征是有强烈的促结缔组织增生反应，并形成丰富的网状纤维。然而，10天后，在RG2肾包膜下肿瘤（SRT）的中心，这种反应仅在肿瘤血管附近发现，而在所有研究的病例中，RN6 SRT在肿瘤细胞周围均显示有网状纤维。移植实验表明，恶性RN6和RG2球体是研究体内和体外培养中三维早期肿瘤生长的合适工具。肾包膜下球体移植模型操作简单且可重复性好。与皮下肿瘤相比，SRT模型在肿瘤生长早期具有优势，因为肿瘤在大体上清晰可见，并且可以很容易地进行适当的形态计量学分析，这表明该模型可能适用于实验性化疗和放疗研究。
