一种用于可视化小鼠原位神经血管界面的方案。

A protocol for visualization of murine in situ neurovascular interfaces.

机构信息

Neuro-Oncology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA; Surgical Neurology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA.

Neurosimplicity LLC, Shrewsbury, NJ 07702, USA.

出版信息

STAR Protoc. 2023 Sep 15;4(3):102367. doi: 10.1016/j.xpro.2023.102367. Epub 2023 Jun 19.

DOI:10.1016/j.xpro.2023.102367

PMID:37339049

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10511866/

Abstract

Mapping cranial vasculature and adjacent neurovascular interfaces in their entirety will enhance our understanding of central nervous system function in any physiologic state. We present a workflow to visualize in situ murine vasculature and surrounding cranial structures using terminal polymer casting of vessels, iterative sample processing and image acquisition, and automated image registration and processing. While this method does not obtain dynamic imaging due to mouse sacrifice, these studies can be performed before sacrifice and processed with other acquired images. For complete details on the use and execution of this protocol, please refer to Rosenblum et al..

摘要

全面描绘颅脑血管和邻近的神经血管界面将有助于我们理解中枢神经系统在任何生理状态下的功能。我们提出了一种工作流程，使用血管末端聚合物铸型、迭代样本处理和图像采集以及自动图像配准和处理来可视化原位小鼠血管和周围颅骨结构。虽然由于小鼠牺牲而无法获得动态成像，但这些研究可以在牺牲前进行，并与其他获得的图像一起处理。有关此方案的使用和执行的完整详细信息，请参阅 Rosenblum 等人的文章。