利用双光子显微镜对移植的小鼠骨髓细胞进行活体成像的方案。
Protocol for live imaging of transferred mouse bone marrow cells by two-photon microscopy.
机构信息
Department of Immunology and Cell Biology, Graduate School of Medicine and Frontier Biosciences, Osaka University, Suita, Osaka 565-0871, Japan.
Department of Immunology and Cell Biology, Graduate School of Medicine and Frontier Biosciences, Osaka University, Suita, Osaka 565-0871, Japan.
出版信息
STAR Protoc. 2023 Dec 15;4(4):102654. doi: 10.1016/j.xpro.2023.102654. Epub 2023 Oct 20.
The in situ behavior of living cells can be visualized by two-photon microscopy. Here, we present a protocol for the live imaging of transferred mouse bone marrow cells by two-photon microscopy. We describe steps for staining and injecting target cells into mice, fixing the skull bone to a head holder and stage, and 4D imaging bone marrow using multi-photon microscopy. We then detail procedures for creating images and analyzing cells. For complete details on the use and execution of this protocol, please refer to Sudo et al. (2021)..
利用双光子显微镜可以观察活细胞的原位行为。在这里，我们提供了一个使用双光子显微镜对转染的小鼠骨髓细胞进行活体成像的方案。我们描述了对目标细胞进行染色和注射、将颅骨固定在头部固定器和载物台上，以及使用多光子显微镜对骨髓进行 4D 成像的步骤。然后，我们详细介绍了创建图像和分析细胞的过程。如需详细了解本方案的使用和执行，请参考 Sudo 等人（2021 年）。
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