Hospices Civils de Lyon, Immunology Laboratory, Hôpital E. Herriot, Lyon France; Université de Lyon, EA 7426 "Pathophysiology of Injury-Induced Immunosuppression", Université Claude Bernard Lyon_1, Lyon, France.
Research & Development Department, bioMérieux S.A., Marcy l'Etoile, France.
Cytokine. 2023 Sep;169:156263. doi: 10.1016/j.cyto.2023.156263. Epub 2023 Jun 19.
In sepsis, personalized immunotherapy is being evaluated as a means of restoring immune function in the most severely affected patients. Biomarkers play a crucial role in this process, as there are no clear clinical indicators of immune dysfunction. Functional testing is considered a gold standard for assessing immune function, but this approach faces analytical challenges in terms of clinical implementation. The use of technician-dependent, time-consuming, home-made protocols often leads to poor standardization. This study represents the first beta testing of a fully automated interferon-γ release assay (IGRA) for monitoring the functionality of antigen-independent T lymphocytes. We observed a significant decrease in IFN-γ release capacity, which was associated with typical alterations in immunological cellular parameters (such as low mHLA-DR expression and decreased CD8 T lymphocyte count), in 22 patients with septic shock. Since the test is performed using whole blood and requires no technician intervention, with results available within 4 h, it may offer new possibilities for monitoring patients with immune alterations in routine clinical conditions. Further investigations in larger cohorts of patients are now needed to validate its clinical potential.
在脓毒症中，个性化免疫疗法正被评估为恢复病情最严重患者免疫功能的一种手段。生物标志物在这一过程中发挥着关键作用，因为目前尚无明确的免疫功能障碍的临床指标。功能测试被认为是评估免疫功能的金标准，但这种方法在临床实施方面存在分析挑战。使用依赖技术人员、耗时且自制的方案往往导致标准化程度较差。本研究代表了一种全自动干扰素-γ释放试验（IGRA）用于监测抗原非依赖性 T 淋巴细胞功能的首次β测试。我们观察到 22 例脓毒性休克患者的 IFN-γ 释放能力显著下降，这与免疫细胞参数的典型改变有关（例如低 MHC-DR 表达和 CD8 T 淋巴细胞计数减少）。由于该检测使用全血进行，无需技术人员干预，结果可在 4 小时内获得，因此它可能为监测常规临床条件下免疫改变的患者提供新的可能性。现在需要在更大的患者队列中进行进一步的研究来验证其临床潜力。
