通过酶联免疫吸附测定法快速诊断中枢神经系统布鲁氏菌病

Rapid diagnosis of central nervous system brucellosis by ELISA.

作者信息

Araj G F, Lulu A R, Saadah M A, Mousa A M, Strannegard I L, Shakir R A

出版信息

J Neuroimmunol. 1986 Sep;12(3):173-82. doi: 10.1016/s0165-5728(86)80001-7.

Abstract

The diagnosis of central nervous system (CNS) brucellosis was made by determining Brucella-specific IgG, IgM and IgA in cerebrospinal fluid (CSF) specimens, using an enzyme-linked immunosorbent assay (ELISA) method. A total of 68 CSF specimens including 10 from patients with brucellosis of the nervous system, 4 with Brucella infection but without CNS involvement, 38 with meningitis other than due to Brucella and 16 with no meningitis were studied. Of the 10 CSF specimens from patients with CNS brucellosis, Brucella-specific IgG was detected in all 10, IgM in 7 and IgA in 8. The 10 patients were also seropositive for anti-Brucella IgG and IgM, and 9 were seropositive for IgA. No Brucella-specific IgG, IgM or IgA was detected in the CSF of 4 patients with brucellosis but without CNS involvement, even though the blood of these patients had high titres of anti-Brucella IgG, IgM and IgA. In addition, none of the CSF specimens from the 38 patients with meningitis other than due to Brucella, or from the 16 patients without meningitis, had detectable anti-Brucella antibodies. This study indicates that the Brucella ELISA on CSF is a reliable, rapid, sensitive and specific assay in the diagnosis of CNS brucellosis.

摘要

通过酶联免疫吸附测定（ELISA）方法检测脑脊液（CSF）标本中的布鲁氏菌特异性IgG、IgM和IgA，从而做出中枢神经系统（CNS）布鲁氏菌病的诊断。共研究了68份脑脊液标本，其中10份来自神经系统布鲁氏菌病患者，4份来自布鲁氏菌感染但无CNS受累患者，38份来自非布鲁氏菌引起的脑膜炎患者，16份来自无脑膜炎患者。在10份CNS布鲁氏菌病患者的脑脊液标本中，全部10份均检测到布鲁氏菌特异性IgG，7份检测到IgM，8份检测到IgA。这10名患者抗布鲁氏菌IgG和IgM血清学也呈阳性，9名患者IgA血清学呈阳性。4名布鲁氏菌病但无CNS受累患者的脑脊液中未检测到布鲁氏菌特异性IgG、IgM或IgA，尽管这些患者血液中抗布鲁氏菌IgG、IgM和IgA滴度很高。此外，38例非布鲁氏菌引起的脑膜炎患者或16例无脑膜炎患者的脑脊液标本中均未检测到抗布鲁氏菌抗体。本研究表明，脑脊液布鲁氏菌ELISA在CNS布鲁氏菌病诊断中是一种可靠、快速、灵敏且特异的检测方法。