布鲁氏菌病 ELISA IgG 和 IgM 在菌血症和非菌血症布鲁氏菌病患者中的诊断价值
Diagnostic value of Brucella ELISA IgG and IgM in bacteremic and non-bacteremic patients with brucellosis.
作者信息
Osoba A O, Balkhy H, Memish Z, Khan M Y, Al-Thagafi A, Al Shareef B, Al Mowallad A, Oni G A
机构信息
Department of Microbiology, King Khalid National Guard Hospital, Jeddah, Saudi Arabia.
出版信息
J Chemother. 2001 Apr;13 Suppl 1:54-9. doi: 10.1080/1120009x.2001.11782330.
The diagnostic value of Brucella ELISA IgG and IgM has been evaluated in patients with brucellosis. Serum samples and blood cultures were collected from 83 patients with brucellosis. The sera were tested by Brucella ELISA for Brucella IgM and IgG antibodies. All 44 controls were negative for IgG and IgM. Brucella melitensis was isolated from blood cultures of 30/83 (36.1%) patients. Among the 30 bacteremic patients, 24 (80%) had an increased IgM titer of > or = 200. Of the 53 non-bacteremic patients, 41 had IgM titer > or = 200, while 22 had IgG titer of > or = 1,600. The ELISA IgM and IgG tests achieved a specificity and sensitivity of 100% and 96% respectively, while the positive and negative predictive values were 100% and 94% respectively. The Brucella ELISA is a reliable and sensitive test in the diagnosis of brucellosis. The test is rapid, easy to perform and can be automated.
已经对布鲁氏菌病患者中布鲁氏菌酶联免疫吸附测定（ELISA）IgG和IgM的诊断价值进行了评估。从83例布鲁氏菌病患者中采集了血清样本和血培养物。通过布鲁氏菌ELISA检测血清中的布鲁氏菌IgM和IgG抗体。所有44名对照的IgG和IgM均为阴性。从83例患者中的30例（36.1%）血培养物中分离出羊种布鲁氏菌。在这30例菌血症患者中，24例（80%）的IgM滴度升高至≥200。在53例非菌血症患者中，41例IgM滴度≥200，22例IgG滴度≥1600。ELISA IgM和IgG检测的特异性和敏感性分别达到100%和96%，阳性预测值和阴性预测值分别为100%和94%。布鲁氏菌ELISA在布鲁氏菌病诊断中是一种可靠且敏感的检测方法。该检测快速、易于操作且可实现自动化。
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