Wechbanjong N, McMillan P J, Dalgleish A E
Am J Anat. 1979 Apr;154(4):477-84. doi: 10.1002/aja.1001540403.
Calcitonin-containing cells in serial, 6-micrometer sections of the thyroid glands of Swiss Webster mice, at 1 day, 2 weeks, 4 weeks and 8 weeks of age, were demonstrated by an immunoperoxidase method, using antiserum to human calcitonin. C-cell nuclei were counted in every sixth section of both left and right lobes. The average number of C-cells counted in the thyroid glands of 8-week-old animals was 18-fold, 5.5-fold and 2.5-fold greater than the number observed in 1-day, 2-week and 4-week-old animals, respectively. C-cell concentration was found to be greatest in 4-week-old mice. Mitoses of C-cells were observed in animals which were 1 day, 2 weeks and four weeks old. No mitotic figures were seen in 8-week-old animals. A few C-cells were seen in close association with neurons. The volume of the thyroid glands of 8-week-old animals was about 14-, 4- and 3-fold greater than the volume in the 1-day-old, 2-week-old and 4-week-old mice, respectively. These changes in the C-cell population during development provide a model for the study of C-cell proliferation and storage of calcitonin.
采用抗人降钙素抗血清，通过免疫过氧化物酶法对1日龄、2周龄、4周龄和8周龄瑞士韦伯斯特小鼠甲状腺连续6微米切片中的降钙素细胞进行了检测。在左右叶的每第六个切片中对C细胞核进行计数。8周龄动物甲状腺中计数的C细胞平均数量分别比1日龄、2周龄和4周龄动物中观察到的数量多18倍、5.5倍和2.5倍。发现4周龄小鼠的C细胞浓度最高。在1日龄、2周龄和4周龄的动物中观察到了C细胞的有丝分裂。在8周龄动物中未见到有丝分裂图像。观察到一些C细胞与神经元紧密相连。8周龄动物的甲状腺体积分别比1日龄、2周龄和4周龄小鼠的甲状腺体积大14倍、4倍和3倍。发育过程中C细胞群体的这些变化为研究C细胞增殖和降钙素储存提供了一个模型。
