Treilhou-Lahille F, Cressent M, Taboulet J, Moukhtar M S
C R Seances Acad Sci D. 1979 Sep 17;289(4):421-3.
Calcitonin was revealed by means of an indirect immunohistochemical method using a serum against synthetic human calcitonin. After the use of the Bouin-Hollande fixative liquid, the hormone was detected in cells located in the central part of the thyroïdian wings. They are few at the 18th day of fetal life, their number increases regularly until reaching ten times the initial number at birth. The immunological reaction is negative if only aldehyde fixatives are used.
采用抗合成人降钙素血清，通过间接免疫组织化学方法检测到降钙素。使用布安 - 霍兰德固定液后，在甲状腺叶中央部分的细胞中检测到该激素。在胎儿期第18天时数量较少，其数量不断增加，直至出生时达到初始数量的十倍。如果仅使用醛类固定剂，免疫反应则为阴性。
