Zheng Jiangtao, Sun Lili, Wang Dongmei, He Lin, Du Weijun, Guo Shujin, Wang Lixiang
College of Agronomy, Shanxi Agricultural University, Taigu, China.
State Key Laboratory of Crop Stress Adaptation Improvement, School of Life Sciences, Henan University, Kaifeng, China.
Front Plant Sci. 2023 Jun 5;14:1172354. doi: 10.3389/fpls.2023.1172354. eCollection 2023.
Legume-rhizobial symbiotic nitrogen fixation is the most efficient nitrogen assimilation system in the ecosystem. In the special interaction between organ-root nodules, legumes supply rhizobial carbohydrates for their proliferation, while rhizobials provide host plants with absorbable nitrogen. Nodule initiation and formation require a complex molecular dialogue between legumes and rhizobia, which involves the accurate regulation of a series of legume genes. The CCR4-NOT complex is a conserved multi-subunit complex with functions regulating gene expression in many cellular processes. However, the functions of the CCR4-NOT complex in rhizobia-host interactions remain unclear. In this study, we identified seven members of the family in soybean and further classified them into three subgroups. Bioinformatic analysis showed that shared relatively conserved motifs and gene structures in each subgroup, while there were significant differences between in the different subgroups. Expression profile analysis indicated that may be involved in nodulation in soybean, as most of them were induced by Rhizobium infection and highly expressed in nodules. We further selected to clarify the biological function of these genes in soybean nodulation. Interestingly, we found that either overexpression or down-regulation of by RNAi or CRISPR/Cas9 gene editing would suppress the number of nodules in soybean. Intriguingly, alterations in the expression of repressed the expression of genes in the Nod factor signaling pathway. This research provides new insight into the function of the family in legumes and reveals to be a potent gene for regulating symbiotic nodulation.
豆科植物-根瘤菌共生固氮是生态系统中最有效的氮同化系统。在根瘤这种特殊的器官-根系相互作用中，豆科植物为根瘤菌的增殖提供碳水化合物，而根瘤菌为宿主植物提供可吸收的氮。根瘤的起始和形成需要豆科植物和根瘤菌之间复杂的分子对话，这涉及一系列豆科植物基因的精确调控。CCR4-NOT复合体是一种保守的多亚基复合体，在许多细胞过程中具有调节基因表达的功能。然而，CCR4-NOT复合体在根瘤菌与宿主相互作用中的功能仍不清楚。在本研究中，我们在大豆中鉴定出该家族的七个成员，并进一步将它们分为三个亚组。生物信息学分析表明，每个亚组中的成员具有相对保守的基序和基因结构，而不同亚组之间的成员存在显著差异。表达谱分析表明，这些成员可能参与大豆的结瘤过程，因为它们中的大多数受根瘤菌感染诱导并在根瘤中高表达。我们进一步选择这些成员来阐明这些基因在大豆结瘤中的生物学功能。有趣的是，我们发现通过RNAi或CRISPR/Cas9基因编辑过表达或下调这些成员都会抑制大豆根瘤的数量。有趣的是，这些成员表达水平的改变会抑制根瘤因子信号通路中基因的表达。本研究为CCR4-NOT家族在豆科植物中的功能提供了新的见解，并揭示了这些成员是调节共生结瘤的重要基因。
