Askari Badouei Mahdi, Taban Haniye, Nemati Ali, Fernando Dos Santos Luis
Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.
Department of Microbiology, Faculty of Biology, Damghan Branch, Islamic Azad University, Semnan, Iran.
Vet Res Forum. 2023;14(5):267-274. doi: 10.30466/vrf.2022.550618.3416. Epub 2023 May 15.
The present study reported the first serotyping (O:H typing) data documented in Shiga toxin-producing (STEC) strains of animal origin in Iran in isolates recovered between 2008 to 2016. A total number of 75 STEC strains previously isolated from fecal samples of cattle, sheep, goats, pigeons, humans, and deer were assessed by different polymerase chain reaction (PCR) assays detecting the major virulence genes of STEC and phylogroups. Then, the strains were tested for the 16 important O-groups by PCR. Finally, twenty strains were selected for H-genotyping by PCR plus sequencing. The predominant serogroup was O113 which was detected in nine isolates (five cattle, 55.50%; two goats, 22.20%; two red deer, 22.20%) followed by O26 (3/3, 100%) in cattle, O111 (3/3, 100%) in cattle, O5 (3/3, 100%) in sheep, O63 (1/1, 100%) in pigeon, O75 (2/2, 100%) in pigeons, and O128 in goats (2/3, 66.60%) and pigeon (1/3, 33.30%). The most important recognized serotypes were O113:H21 in cattle (2/3) and goat (1/3), O113:H4 in red deer (1/1), O111:H8 in calves (2/2), O26:H11 in calve (1/1), O128:H2 in goats (2/3) and pigeon (1/3), and O5:H19 in sheep (3/3). One cattle strain carrying 1, 2, , and genes belonged to O26:H29 serotype. Most strains with determined O-groups were from the bovine source that highlighted the importance of cattle as reservoirs of potentially pathogenic serovars. The present study suggested that the top seven non-O157 serogroups should be assessed along with O157 in all future research and clinical diagnostics of STEC in Iran.
本研究报告了2008年至2016年间在伊朗分离出的动物源产志贺毒素（STEC）菌株中的首批血清分型（O:H分型）数据。通过不同的聚合酶链反应（PCR）检测方法，对先前从牛、羊、山羊、鸽子、人类和鹿的粪便样本中分离出的75株STEC菌株进行了评估，以检测STEC的主要毒力基因和系统发育群。然后，通过PCR对这些菌株进行16种重要O群的检测。最后，选择20株菌株通过PCR加测序进行H分型。优势血清群是O113，在9株分离物中检测到（5头牛，55.50%；2只山羊，22.20%；2只马鹿，22.20%），其次是牛中的O26（3/3，100%）、牛中的O111（3/3，100%）、羊中的O5（3/3，100%）、鸽子中的O63（1/1，100%）、鸽子中的O75（2/2，100%）以及山羊（2/3，66.60%）和鸽子（1/3，33.30%）中的O128。最重要的公认血清型是牛（2/3）和山羊（1/3）中的O113:H21、马鹿（1/1）中的O113:H4、犊牛（2/2）中的O111:H8、犊牛（1/1）中的O26:H11、山羊（2/3）和鸽子（1/3）中的O128:H2以及羊（3/3）中的O5:H19。一株携带1、2、 和 基因的牛菌株属于O26:H29血清型。大多数确定了O群的菌株来自牛源，这突出了牛作为潜在致病性血清型宿主的重要性。本研究表明在伊朗未来所有关于STEC的研究和临床诊断中应将前七种非O157血清群与O157一起进行评估。
