Department of Microbiology, Immunology and Veterinary Public Health, College of Veterinary Medicine and Agriculture, Addis Ababa University, Bishoftu, Ethiopia.
Department of Veterinary Public Health and Food Safety, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
Foodborne Pathog Dis. 2021 Jan;18(1):1-7. doi: 10.1089/fpd.2020.2830. Epub 2020 Aug 31.
O157 is a Shiga toxin-producing causing disease in humans. Cattle are the primary reservoir of the pathogen. Information regarding the contribution of cattle to diarrheal illnesses in humans through consumption of contaminated beef is scarce in Ethiopia. We collected samples from 240 cattle, 127 beef, and 216 diarrheic patients in Bishoftu town in Ethiopia to assess the occurrence and determine the virulence genes, genetic relatedness, and antimicrobial resistance of O157. O157 was detected in 7.1% of the rectal content samples from cattle in slaughterhouses, in 6.3% ( = 127) of the beef samples, and in 2.8% of the diarrheic patients' stool samples. All isolates were positive for gene, 24 (77%) of them were positive for 2 gene (21 2c and 3 2a), whereas 1 gene was not detected. Molecular typing grouped the isolates into eight pulsed-field gel electrophoresis pulsotypes with three pulsotypes containing isolates from all three sources, one pulsotype containing one isolate from human origin and one isolate from beef. The remaining four pulsotypes contained isolates unique either to beef or to humans. With the exception of 1 multidrug-resistant isolate from beef, which was resistant to 8 antimicrobial drugs, the remaining 30 isolates were susceptible to the 14 antimicrobials tested. In conclusion, the finding of genetically similar isolates in cattle, beef, and humans may indicate a potential transmission of O157 from cattle to humans through beef. However, more robust studies are required to confirm this epidemiological link.
O157 是一种产志贺毒素的 ，可引起人类疾病。牛是该病原体的主要宿主。关于人类通过食用受污染的牛肉从牛中感染腹泻病的信息在埃塞俄比亚很少。我们在埃塞俄比亚比绍夫图镇收集了 240 头牛、127 份牛肉和 216 份腹泻患者的样本，以评估其发生情况并确定 O157 的毒力基因、遗传关系和抗药性。在屠宰场的牛直肠内容物样本中，7.1%（240 份样本中的 18 份）、6.3%（127 份牛肉样本中的 8 份）和 2.8%（216 份腹泻患者粪便样本中的 6 份）检测到 O157。所有分离株均为 基因阳性，其中 24 株（77%）为 2 基因阳性（21 2c 和 3 2a），而 1 基因未检出。分子分型将分离株分为 8 个脉冲场凝胶电泳脉冲型，其中 3 个脉冲型包含来自所有 3 个来源的分离株，1 个脉冲型包含 1 个人源分离株和 1 个牛肉源分离株。其余 4 个脉冲型包含仅来自牛肉或人类的分离株。除了来自牛肉的 1 株多药耐药分离株对 8 种抗菌药物耐药外，其余 30 株分离株对测试的 14 种抗菌药物均敏感。总之，在牛、牛肉和人类中发现遗传上相似的分离株可能表明 O157 从牛向人类通过牛肉传播的潜在风险。然而，需要进行更强大的研究来证实这种流行病学联系。
