Kopparthi Jalajakshi, Chennuru Sreedevi, Rayulu Vukka Chengalva, Nalini Kumari Karumuri, Rani Prameela Devalam
Department of Veterinary Parasitology, College of Veterinary Science, Sri Venkateswara Veterinary University, Tirupati, India.
Department of Veterinary Parasitology, College of Veterinary Science, Sri Venkateswara Veterinary University, Gannavaram, India.
Vet Res Forum. 2023;14(5):295-299. doi: 10.30466/vrf.2022.546812.3351. Epub 2023 May 15.
Implementing hemoprotozoan control strategies in dogs has become difficult because of the co-infections. A multiplex polymerase chain reaction (PCR) was carried out for simultaneous detection of the co-infections of , and from dogs (N = 442) in Andhra Pradesh, South India. The co-infection combinations were classified as (i) + + (BEH), (ii) + (BE), (iii) + (BH) and (iv) + (EH) groups. The parasite-specific multiplex PCR amplified 18S rRNA gene of , and . and gene of . . The age, gender, breed, medium, living condition and region of dogs were studied as risk factors for co-infections using logistic regression model. Among the co-infections, the incidence was 1.81%, 9.28%, 0.69% and 0.90% for BEH, BE, BH and EH infections, respectively. Young age (< one year), females, mongrels, rural dogs, kennel dogs and presence of ticks were the identified risk factors for overall prevalence of tick-borne pathogens. The incidence of infection was less in rainy season, especially in dogs with a previous acaricidal treatment. The study concludes that the multiplex PCR assay could simultaneously detect natural co-infections in dogs, emphasizing the need for the assay in epidemiological studies to reveal the real pattern of pathogens and select pathogen-specific treatment protocols.
由于存在合并感染，在犬类中实施血液原虫控制策略变得困难。对印度南部安得拉邦442只犬进行了多重聚合酶链反应（PCR），以同时检测 、 和 的合并感染情况。合并感染组合分为：（i） + + （BEH）、（ii） + （BE）、（iii） + （BH）和（iv） + （EH）组。寄生虫特异性多重PCR扩增了 、 和 的18S rRNA基因，以及 的 和 基因。使用逻辑回归模型研究犬的年龄、性别、品种、饲养方式、生活条件和地区作为合并感染的危险因素。在合并感染中，BEH、BE、BH和EH感染的发生率分别为1.81%、9.28%、0.69%和0.90%。幼犬（<1岁）、雌性、杂种犬、农村犬、犬舍犬以及蜱的存在是蜱传播病原体总体流行的确定危险因素。雨季感染发生率较低，尤其是之前接受过杀螨治疗的犬。该研究得出结论，多重PCR检测可同时检测犬类中的自然合并感染，强调该检测在流行病学研究中对于揭示病原体真实模式和选择病原体特异性治疗方案的必要性。
