Li Wenlong, Sun Beibei, Li Huijun, An Zhen, Li Juan, Jiang Jing, Song Jie, Wu Weidong
Henan International Laboratory for Air Pollution Health Effects and Intervention, School of Public Health, Xinxiang Medical University, Xinxiang, Henan Province 453003, China; Institute of Infectious Disease Prevention and Control, Zhengzhou Center for Disease Control and Prevention, Zhengzhou, Henan Province 450000, China.
Henan International Laboratory for Air Pollution Health Effects and Intervention, School of Public Health, Xinxiang Medical University, Xinxiang, Henan Province 453003, China.
Ecotoxicol Environ Saf. 2023 Jun 19;262:115156. doi: 10.1016/j.ecoenv.2023.115156.
Fine particulate matter (PM) is the primary environmental stressor and a significant threat to public health. However, the effect of PM exposure on human nasal microbiota and its pathophysiological implication remain less understood. This study aimed to explore the associations of PM exposure with indices of nasal microbiota and biomarkers of nasal inflammation and oxidative stress. We conducted a panel study with 75 students in Xinxiang, Henan Province, China, from September to December 2017. Biomarkers of nasal inflammation and oxidative stress including interleukin-6 (IL-6), IL-8, tumor necrosis factor-α (TNF-α), 8-epi-prostaglandin F2 alpha (8-epi-PGF2α) and indices of nasal microbiota diversity and phenotypes were measured. Linear mixed-effect models and bioinformatic analyses were performed to assess the association of PM concentrations with the abovementioned biomarkers and indices. It was found that per 1 μg/m increase in PM was associated with increments of 13.15% (95 % CI: 5.53-20.76 %) and 78.98 % (95 % CI: 21.61-136.36 %) in TNF-α on lag2 and lag02. Indices of microbial diversity and phenotypes including equitability, Shannon index, biofilm forming, and oxidative stress tolerant decreased to different extent with the increment in PM. Notably, thirteen differential microbes in Clostridia, Bacilli, and Gammaproteobacteria classes were recognized as keystone taxa and eight of them were associated with TNF-α, IL-6, or 8-epi-PGF2α. Moreover, environmental adaptation was the most critical functional pathway of nasal microbiota associated with PM exposure. In summary, short-term exposure to PM is associated with nasal inflammation, microbiota diversity reduction, and the microbiota phenotype alterations.
细颗粒物（PM）是主要的环境应激源，对公众健康构成重大威胁。然而，暴露于PM对人类鼻腔微生物群的影响及其病理生理意义仍鲜为人知。本研究旨在探讨PM暴露与鼻腔微生物群指标以及鼻腔炎症和氧化应激生物标志物之间的关联。2017年9月至12月，我们在中国河南省新乡市对75名学生进行了一项专题研究。测量了鼻腔炎症和氧化应激的生物标志物，包括白细胞介素-6（IL-6）、IL-8、肿瘤坏死因子-α（TNF-α）、8-表前列腺素F2α（8-epi-PGF2α）以及鼻腔微生物群多样性和表型指标。采用线性混合效应模型和生物信息学分析来评估PM浓度与上述生物标志物和指标之间的关联。结果发现，PM每增加1μg/m，TNF-α在滞后2和滞后02时分别增加13.15%（95%CI：5.53-20.76%）和78.98%（95%CI：21.61-136.36%）。微生物多样性和表型指标，包括公平性、香农指数、生物膜形成和氧化应激耐受性，随着PM的增加而不同程度地降低。值得注意的是，梭菌纲、芽孢杆菌纲和γ-变形菌纲中的13种差异微生物被识别为关键类群，其中8种与TNF-α、IL-6或8-epi-PGF2α相关。此外，环境适应是与PM暴露相关的鼻腔微生物群最关键的功能途径。总之，短期暴露于PM与鼻腔炎症、微生物群多样性降低和微生物体表型改变有关。
