College of Resources and Environmental Science, Beijing Key Laboratory of Biodiversity and Organic Farming, China Agricultural University, Beijing 100193, China; Organic Recycling Institute (Suzhou) of China Agricultural University, Suzhou 215128, China.
College of Resources and Environmental Science, Beijing Key Laboratory of Biodiversity and Organic Farming, China Agricultural University, Beijing 100193, China.
Bioresour Technol. 2023 Sep;384:129339. doi: 10.1016/j.biortech.2023.129339. Epub 2023 Jun 19.
The aim of this study was to assess the effect of polylactic acid (PLA) on microbial community composition and core metabolism pathways in food waste (FW) composting. The presence of PLA negatively influenced microbial community richness and decreased respectively the abundance of Bacillus, Halocella and Cellvibrio at mesophilic, thermophilic and mature phases. Analysis of microbial metabolism at the gene level help to understand the mechanism in co-composting with FW and PLA. The expression of core functional genes related to lactide metabolism was stimulated by PLA degradation at thermophilic and mature phases. The sum of absolute abundance of functional genes that involved in first and second carbon oxidation of tricarboxylic acid cycle was decreased due to the existence of PLA. The transformation between 2-Oxoglutarate and Succinyl-CoA were interfered in thermophilic phase, which might result in the lower germination index in PLA group (115%) than that in control (186%).
本研究旨在评估聚乳酸（PLA）对食品垃圾（FW）堆肥中微生物群落组成和核心代谢途径的影响。PLA 的存在对微生物群落丰富度产生负面影响，分别降低了中温、高温和成熟阶段芽孢杆菌、盐单胞菌和纤维弧菌的丰度。对 FW 和 PLA 共堆肥过程中微生物代谢的基因水平分析有助于理解其作用机制。在高温和成熟阶段，PLA 降解刺激了与丙交酯代谢相关的核心功能基因的表达。由于 PLA 的存在，参与三羧酸循环第一和第二碳氧化的功能基因的绝对丰度总和减少。在高温阶段，2-氧戊二酸和琥珀酰辅酶 A 之间的转化受到干扰，这可能导致 PLA 组（115%）的发芽指数低于对照组（186%）。
