Vector Biology Department, Liverpool School of Tropical Medicine, Liverpool, UK.
Sci Rep. 2023 Jun 21;13(1):10055. doi: 10.1038/s41598-023-35958-z.
The blood feeding performance of female mosquitoes directly impacts their ability to transmit malaria. Yet their host seeking and blood feeding behaviours in the presence of insecticide-treated nets (ITNs) are still poorly understood. This work explores how both insecticide resistant and susceptible Anopheles gambiae s.l. mosquitoes interact with pyrethroid nets (PermaNet 2.0 or Olyset net) or an untreated net (UTN) while attempting to blood feed on a human arm. Regardless of mosquito resistance status, the ITNs did not efficiently prevent host searching but reduced blood feeding success by 34.1 (29.31-38.95) %. The Permanet and Olyset net reduced to 227.5 (208.19-246.77) sec and 235.9 (214.03-257.74) sec the average blood feeding duration from 369.9 (342.78-397.04) sec with the UTN. The ingested blood volume was on average 22% lower for all mosquitoes exposed to insecticide. When feeding through ITNs, the blood volume flow rate of the susceptible strain increased by 35%, but no significant difference was found in the resistant strain. Thus, whilst the presence of the insecticide in ITNs reduced mosquito blood feeding success and blood volume, the mosquito's ability to respond by accelerating her rate of blood ingestion may further reduce the impact of ITNs on resistant mosquitoes.
雌性蚊子的吸血性能直接影响其传播疟疾的能力。然而，在使用杀虫剂处理过的蚊帐（ITN）的情况下，它们的宿主寻找和吸血行为仍未得到很好的理解。这项工作探讨了抗药性和敏感性的冈比亚按蚊（Anopheles gambiae s.l.）如何与拟除虫菊酯网（PermaNet 2.0 或 Olyset 网）或未处理的网（UTN）相互作用，同时试图在人的手臂上吸血。无论蚊子的抗药性如何，ITN 并不能有效地阻止宿主的寻找，但会使吸血成功率降低 34.1%（29.31-38.95）%。PermaNet 和 Olyset 网将平均吸血时间从使用 UTN 的 369.9 秒（342.78-397.04）缩短到 227.5 秒（208.19-246.77）和 235.9 秒（214.03-257.74）。与使用 UTN 相比，所有接触杀虫剂的蚊子的吸血量平均减少了 22%。当通过 ITN 吸血时，敏感株的吸血量流量增加了 35%，但在抗性株中没有发现显著差异。因此，尽管 ITN 中的杀虫剂降低了蚊子的吸血成功率和吸血量，但蚊子通过加速吸血速度来应对的能力可能会进一步降低 ITN 对抗性蚊子的影响。
