Institute of Disaster and Emergency Medicine, Tianjin University, Tianjin, 300072, PR China.
Key Laboratory of Industrial Microbiology, Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology, China International Science and Technology Cooperation Base of Food Nutrition/Safety and Medicinal Chemistry, Tianjin, 300457, PR China.
Carbohydr Res. 2023 Aug;530:108875. doi: 10.1016/j.carres.2023.108875. Epub 2023 Jun 16.
Chitotriose (CTS), the hydrolysate of chitosan, is readily soluble in water because of the shorter chain lengths of the oligomers and the free amino groups in the d-glucosamine units. In the current study, we report the synthesis of novel conjugate vaccine Tn-BSA-CTS with chitotriose as built-in adjuvant, along with an evaluation of the effect of adjuvant chitotriose (CTS). Immunological evaluations of the resultant conjugate vaccine revealed that Tn-BSA-CTS could provoke the highest titers of IgG antibodies (102,400). The Tn-BSA-CTS conjugate remarkably enhanced both humoral and cellular immunity. The obtained results demonstrate the potential of CTS as a novel vaccine adjuvant in the development of antitumor vaccine and the covalent linkage of tumor vaccine to CTS might be available strategy to increase the efficacy against cancer.
壳三糖（CTS）是壳聚糖的水解产物，由于低聚物的链长较短以及 D-葡萄糖胺单元中的游离氨基，因此其在水中易溶解。在本研究中，我们报告了新型结合疫苗 Tn-BSA-CTS 的合成，其中壳三糖（CTS）作为内置佐剂，同时评估了佐剂壳三糖（CTS）的作用。对所得结合疫苗的免疫学评估表明，Tn-BSA-CTS 可以引发最高效价的 IgG 抗体（102400）。Tn-BSA-CTS 缀合物显著增强了体液和细胞免疫。所得结果表明 CTS 作为新型肿瘤疫苗佐剂具有潜力，并且将肿瘤疫苗与 CTS 共价连接可能是提高抗癌疗效的可行策略。
