Key Laboratory for Green Organic Synthesis and Application of Hunan Province, Key Laboratory of Environmentally Friendly Chemistry and Applications of Ministry of Education, College of Chemistry, Xiangtan University, Xiangtan, 411105, PR China.
Henan Key Laboratory of Organic Functional Molecule and Drug Innovation, Collaborative Innovation Center of Henan Province for Green Manufacturing of Fine Chemicals, Key Laboratory of Green Chemical Media and Reactions, Ministry of Education, School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang, 453007, PR China.
Talanta. 2023 Dec 1;265:124815. doi: 10.1016/j.talanta.2023.124815. Epub 2023 Jun 16.
Cancer is one of the major diseases that seriously endanger the health of all mankind. Accurate diagnosis of early cancer is the most promising way to reduce cancer harm and improve patient survival. However, many developed fluorescent probes for cancer imaging only have the function of identifying one marker, which cannot meet the needs of accurate diagnosis. Here, a fluorescent nanoprobe (CPH@ZIF-90) utilizing ZIF-90 to encapsulate SO-sensitive dye (CPH) is synthesized for the sequential detection of ATP and SO. The nanoprobe first interacts with ATP to release CPH, thus increasing the fluorescence at 685 nm and realizing the near-infrared (NIR) fluorescence detection of ATP. Then, SO acts on the released CPH through nucleophilic addition, affecting the π-conjugated structure of CPH and resulting in enhanced fluorescence at 580 nm. CPH@ZIF-90 exhibits satisfactory sensitivity and selectivity for sequential detection of ATP and SO. Excitedly, CPH@ZIF-90 can sequentially image the endogenous ATP and SO in cells, showing sensitive fluorescence changes in dual channels (red and green). Due to the NIR emission properties of CPH@ZIF-90 and its ability to enrich in tumor, it is applied to monitor ATP and SO in mice and distinguish normal mice from tumor mice. The ability of CPH@ZIF-90 to sequentially detect two cancer-related biomarkers makes it provide meaningful assistance in accurate early diagnosis of cancer.
癌症是严重威胁全人类健康的主要疾病之一。准确诊断早期癌症是降低癌症危害、提高患者生存率最有希望的途径。然而，许多用于癌症成像的荧光探针仅具有识别一种标志物的功能，无法满足准确诊断的需求。在这里，我们合成了一种利用 ZIF-90 封装 SO 敏感染料（CPH）的荧光纳米探针（CPH@ZIF-90），用于 ATP 和 SO 的顺序检测。该纳米探针首先与 ATP 相互作用释放 CPH，从而增加 685nm 处的荧光强度，实现对 ATP 的近红外（NIR）荧光检测。然后，SO 通过亲核加成作用作用于释放的 CPH，影响 CPH 的π-共轭结构，导致 580nm 处的荧光增强。CPH@ZIF-90 对 ATP 和 SO 的顺序检测表现出令人满意的灵敏度和选择性。令人兴奋的是，CPH@ZIF-90 可以在细胞中顺序成像内源性 ATP 和 SO，在双通道（红色和绿色）中显示出敏感的荧光变化。由于 CPH@ZIF-90 的 NIR 发射特性及其在肿瘤中的富集能力，它被应用于监测小鼠中的 ATP 和 SO，并区分正常小鼠和肿瘤小鼠。CPH@ZIF-90 顺序检测两种与癌症相关的生物标志物的能力为癌症的准确早期诊断提供了有意义的帮助。
