Key Laboratory for Green Organic Synthesis and Application of Hunan Province, Key Laboratory of Environmentally Friendly Chemistry and Applications of Ministry of Education, College of Chemistry, Xiangtan University, Xiangtan, 411105, People's Republic of China.
Henan Key Laboratory of Organic Functional Molecule and Drug Innovation, Collaborative Innovation Center of Henan Province for Green Manufacturing of Fine Chemicals, Key Laboratory of Green Chemical Media and Reactions, Ministry of Education, School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang, 453007, People's Republic of China.
Mikrochim Acta. 2021 Aug 4;188(9):287. doi: 10.1007/s00604-021-04953-4.
A near-infrared (NIR) fluorescence nanoprobe named RhI-DOX@ZIF-90 has been synthesized by wrapping the guest molecule (RhI and DOX) into ZIF-90 framework. The nanoprobe itself is non-fluorescent and the drug (DOX) is inactive. Upon the addition of ATP, the structure of RhI-DOX@ZIF-90 is degraded. The fluorescence of RhI is recovered and DOX is released. The nanoprobe can detect ATP with high sensitivity and selectivity. There is good linear relationship between the nanoprobe and ATP concentration from 0.25 to 10 mM and the detection limit is 0.10 mM. The nanoprobe has the ability to monitor the change of ATP level in living cells and DOX is released inducing apoptosis of cancer cells. RhI-DOX@ZIF-90 is capable of targeting mitochondria, which provides a basis for improving the efficiency of drug delivery by mitochondrial administration. In particular, the nanoprobe is preferentially accumulated in the tumor sites and detect ATP in tumor mice by fluorescence imaging using near-infrared fluorescence. At the same time, DOX can be released accurately in tumor sites and have good anti-tumor efficiency. So, this nanoprobe is a reliable tool to realize early diagnosis of cancer and improve effect of anticancer drug.
一种名为 RhI-DOX@ZIF-90 的近红外（NIR）荧光纳米探针通过将客体分子（RhI 和 DOX）包裹到 ZIF-90 骨架中合成。纳米探针本身没有荧光，药物（DOX）也没有活性。加入 ATP 后，RhI-DOX@ZIF-90 的结构被降解。RhI 的荧光恢复，DOX 被释放。该纳米探针具有高灵敏度和选择性检测 ATP 的能力。纳米探针与 ATP 浓度在 0.25 至 10 mM 之间呈良好的线性关系，检测限为 0.10 mM。该纳米探针能够监测活细胞中 ATP 水平的变化，并通过诱导癌细胞凋亡释放 DOX。RhI-DOX@ZIF-90 能够靶向线粒体，为通过线粒体给药提高药物输送效率提供了依据。特别是，该纳米探针优先在肿瘤部位积累，并通过近红外荧光荧光成像在肿瘤小鼠中检测 ATP。同时，DOX 可以在肿瘤部位准确释放，具有良好的抗肿瘤效果。因此，该纳米探针是实现癌症早期诊断和提高抗癌药物效果的可靠工具。
