Faculty of Science and Marine Environment, Universiti Malaysia Terengganu, 21030, Terengganu, Malaysia.
BioInspired Device and Tissue Engineering Research Group, Faculty of Engineering, School of Biomedical Engineering and Health Sciences, Universiti Teknologi Malaysia, Skudai 81300, Johor, Malaysia.
Int J Biol Macromol. 2023 Aug 1;245:125494. doi: 10.1016/j.ijbiomac.2023.125494. Epub 2023 Jun 20.
The demand for advanced wound care products is rapidly increasing nowadays. In this study, gellan gum/collagen (GG/C) hydrogel films containing gatifloxacin (GAT) were developed to investigate their properties as wound dressing materials. ATR-FTIR, swelling, water content, water vapor transmission rate (WVTR), and thermal properties were investigated. The mechanical properties of the materials were tested in dry and wet conditions to understand the performance of the materials after exposure to wound exudate. Drug release by Franz diffusion was measured with all samples showing 100 % cumulative drug release after 40 min. Strong antibacterial activities against Staphylococcus aureus and Staphylococcus epidermis were observed for Gram-positive bacteria, while Escherichia coli and Pseudomonas aeruginosa were observed for Gram-negative bacteria. The in-vivo cytotoxicity of GG/C-GAT was assessed by wound contraction in rats, which was 95 % for GG/C-GAT01. Hematoxylin and eosin and Masson's trichrome staining revealed the appearance of fresh full epidermis and granulation tissue, indicating that all wounds had passed through the proliferation phase. The results demonstrate the promising properties of the materials to be used as dressing materials.
如今，人们对高级伤口护理产品的需求正在迅速增长。在这项研究中，开发了含有加替沙星（GAT）的结冷胶/胶原蛋白（GG/C）水凝胶薄膜，以研究其作为伤口敷料材料的性能。通过衰减全反射傅里叶变换红外光谱（ATR-FTIR）、溶胀度、含水量、水蒸气透过率（WVTR）和热性能对其进行了研究。测试了材料在干燥和湿润条件下的机械性能，以了解材料在接触伤口渗出液后的性能。所有样品在 40 分钟后均表现出 100%的累积药物释放，通过 Franz 扩散法进行了药物释放测试。对革兰氏阳性菌（金黄色葡萄球菌和表皮葡萄球菌）表现出强烈的抗菌活性，而对革兰氏阴性菌（大肠杆菌和铜绿假单胞菌）也表现出一定的抗菌活性。通过大鼠伤口收缩评估 GG/C-GAT 的体内细胞毒性，GG/C-GAT01 的收缩率为 95%。苏木精和伊红以及马松三色染色显示出新鲜完整表皮和肉芽组织的外观，表明所有伤口都已通过增殖期。结果表明，这些材料具有用作敷料材料的巨大潜力。
