School of Fundamental Science, Universiti Malaysia Terengganu, 21030 Kuala Terengganu, Terengganu, Darul Iman, Malaysia.
Institute Biotechnology Marine, Universiti Malaysia Terengganu, 21030 Kuala Terengganu, Terengganu, Darul Iman, Malaysia.
Mater Sci Eng C Mater Biol Appl. 2019 Oct;103:109770. doi: 10.1016/j.msec.2019.109770. Epub 2019 May 18.
In this work, the potential of titanium dioxide nanoparticles incorporated gellan gum (GG + TiO-NPs) biofilm as wound dressing material was investigated. The GG + TiO-NPs biofilm was prepared via evaporative casting technique and was characterized using FTIR, XRD, and SEM to study their physiochemical properties. The mechanical properties, swelling and water vapor transmission rate (WVTR) of biofilm was determined to comply with an ideal wound dressing material. In vitro and in vivo wound healing studies was carried out to evaluate the performance of GG + TiO-NPs biofilm. In vitro wound healing was studied on 3 T3 mouse fibroblast cells for cell viability, cell proliferation, and scratch assay. The acridine orange/propidium iodide (AO/PI) staining and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay were used to evaluate the viability of cell and cell proliferation. Cell migration assay was analyzed using Essen BioScience IncuCyteTM Zoom system. In vivo wound healing via open excision wounds model on Sprague Dawley rat was studied within 14 days. The FT-IR spectra of GG + TiO-NPs biofilm show main bands assigned to OH stretching, OH deformation, and TiO stretching modes. XRD pattern of GG + TiO-NPs biofilm suggesting that TiO-NPs was successfully incorporated in biofilm and well distributed on the surface as proved by SEM analysis. The GG + TiO-NPs biofilm shows higher mechanical strength and swelling (3.76 ± 0.11 MPa and 1061 ± 6%) as compared to pure GG film (3.32 ± 0.08 Mpa and 902 ± 6%), respectively. GG + TiO-NPs biofilm shows good antibacterial properties as 9 ± 0.25 mm and 11 ± 0.06 mm exhibition zone was observed against Staphylococcus aureus and Escherichia coli bacteria, respectively. While no exhibition zone was obtained for pure GG biofilm. GG + TiO-NPs biofilm also demonstrated better cell-to-cell interaction properties, as it's promoted cell proliferation and cell migration to accelerate open excision wound healing on Sprague Dawley rat. The wound treated with GG + TiO-NPs biofilm was healed within 14 days, on the other hand, the wound is still can be seen when it was treated with GG. However, GG and GG + TiO-NPs biofilm show no cytotoxicity effects on mouse fibroblast cells.
在这项工作中，研究了掺入藻酸钙（GG+TiO-NPs）水凝胶的二氧化钛纳米粒子（TiO-NPs）生物膜作为伤口敷料的潜力。通过蒸发铸造技术制备 GG+TiO-NPs 生物膜，并通过傅里叶变换红外光谱（FTIR）、X 射线衍射（XRD）和扫描电子显微镜（SEM）对其物理化学性质进行了表征。为了符合理想的伤口敷料材料，对生物膜的机械性能、溶胀和水蒸气透过率（WVTR）进行了测定。进行了体外和体内伤口愈合研究，以评估 GG+TiO-NPs 生物膜的性能。体外伤口愈合研究在 3T3 小鼠成纤维细胞上进行，以评估细胞活力、细胞增殖和划痕试验。使用吖啶橙/碘化丙啶（AO/PI）染色和 3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四唑溴盐（MTT）测定法评估细胞活力和细胞增殖。使用 Essen BioScience IncuCyteTM Zoom 系统分析细胞迁移试验。通过 Sprague Dawley 大鼠的开放性切除伤口模型研究体内伤口愈合，研究时间为 14 天。GG+TiO-NPs 生物膜的 FT-IR 光谱显示主要带归因于 OH 伸缩、OH 变形和 TiO 伸缩模式。GG+TiO-NPs 生物膜的 XRD 图谱表明，TiO-NPs 成功地掺入生物膜中，并通过 SEM 分析证明在表面上分布良好。与纯 GG 膜（3.32±0.08 MPa 和 902±6%）相比，GG+TiO-NPs 生物膜具有更高的机械强度和溶胀度（3.76±0.11 MPa 和 1061±6%）。GG+TiO-NPs 生物膜表现出良好的抗菌性能，因为对金黄色葡萄球菌和大肠杆菌细菌的抑菌环分别为 9±0.25mm 和 11±0.06mm。而纯 GG 生物膜没有抑菌环。GG+TiO-NPs 生物膜还表现出更好的细胞间相互作用特性，因为它促进了细胞增殖和细胞迁移，从而加速了 Sprague Dawley 大鼠的开放性切除伤口愈合。用 GG+TiO-NPs 生物膜处理的伤口在 14 天内愈合，而用 GG 处理的伤口仍然可见。然而，GG 和 GG+TiO-NPs 生物膜对小鼠成纤维细胞没有细胞毒性作用。
